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Characterization of RING-type ubiquitin SINA E3 ligases and their responsive expression to salt and osmotic stresses in Brassica napus

文献类型： 外文期刊

作者： Fang, Fengyan 1 ; Zhou, Wenlong 1 ; Liu, Yanfeng 1 ; Song, Zhizhong 1 ; Zheng, Songfeng 1 ; Wang, Fei 1 ; Lu, Zeyu 1 ; Qi, Dazhuang 1 ; Li, Bei 1 ; Sun, Nan 1 ; Tang, Xiaoli 1 ; Zhang, Juan 1 ; Zhan, Renhui 5 ; Wang, Limin 1 ; Zhang, Hongxia 1 ;

作者机构： 1.Ludong Univ, Engn Res Inst Agr & Forestry, 186 Hongqizhong Rd, Yantai 264025, Peoples R China

2.Ludong Univ, Coll Agr, 186 Hongqizhong Rd, Yantai 264025, Shandong, Peoples R China

3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Hainan Key Lab Biosafety Monitoring & Mol Breeding, 4 Xueyuan Rd, Haikou 571101, Peoples R China

4.Ludong Univ, Abiot Resistant Plants Univ Shandong, Key Lab Mol Module Based Breeding High Yield, 186 Hongqizhong Rd, Yantai 264025, Shandong, Peoples R China

5.Binzhou Med Univ, Med & Pharm Res Ctr, Yantai 264003, Shandong, Peoples R China

6.Zhaoyuan Shenghui Agr Technol Dev Co Ltd, North Beiyuanzhuang Village, Zhaoyuan 265400, Shandong, Peoples R China

关键词： Brassica napus; SINA; E3 ubiquitin ligase; Salt stress; Osmotic stress

期刊名称：PLANT CELL REPORTS （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0721-7714

年卷期： 2023 年 42 卷 5 期

页码：

收录情况： SCI

摘要： SINA (Seven in absentia) proteins in the subtype of E3 ubiquitin ligase family play a crucial role in plant growth and development. However, their functions in response to salt and osmotic stresses in oil crops are still largely unknown. In this study, a total number of 23 BnaSINAs were identified in the rapeseed genome. Chromosome location and collinear relationship analyses revealed that they were unevenly distributed on 13 chromosomes, and have gone through 22 segmental duplication events under purifying selection. Phylogenetic and gene structural analyses indicated that they belonged to five main groups, and those in the same subgroup showed similar gene structure. All BnaSINAs were predicted to form homo- or heterodimers. Except BnaSINA7, BnaSINA11, BnaSINA17 and BnaSINA18, which lacked the N-terminal RING finger, all BnaSINAs contained a conserved C-terminal SINA domain, a typical structural feature of the RING-type E3 ligase family. Transcriptional expression analyses demonstrated that most BnaSINAs were ubiquitously expressed in roots, stems, leaves, flowers, pods and seeds, and all were responsive to salt and osmotic stresses. Further, yeast two-hybrid and Arabidopsis mutant complementation analyses demonstrated that BnaSINA4 interacted with BnaSINA17 to form heterodimer, and expression of BnaSINA17 in the Arabidopsissina2 mutant restored its growth resistance to salt and osmotic stresses. Our findings provide an important genetic foundation for the functional elucidation of BnaSINAs and a novel gene resource for the breeding of new oil crop cultivars with improved abiotic stress resistance.

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