MOLECULAR CLONING AND FUNCTIONAL ANALYSIS OF FLAVANONE 3-HYDROXYLASE (F3H) GENE IN PASSIFLORA EDULIS SIMS
文献类型： 外文期刊
作者： Ma, Funing 1 ; Wang, Xingmeng 2 ; Wu, Bin 1 ; Xu, Yi 1 ; Huang, Dongmei 1 ; Chen, Ge 3 ; Yang, Liu 3 ; Song, Shun 1 ; Xing, Wenting 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, 4 West Xueyuan Rd, Haikou 571101, Hainan, Peoples R China
2.Anhui Agr Univ, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China
3.Guangxi Acad Agr Sci, 174 East Univ Rd, Nanning 530007, Guangxi, Peoples R China
关键词： Passion fruit; Flavanone 3-hydroxylase gene; qRT-PCR
期刊名称：BANGLADESH JOURNAL OF BOTANY （ 影响因子：0.3； 五年影响因子：0.4 ）
ISSN： 0253-5416
年卷期： 2023 年 52 卷 2 期
页码：
收录情况： SCI
摘要： Flavanone 3-hydroxylase (F3H) plays a crucial role in the biosynthesis of flavonoids. In the present study, one F3H gene (P_edulia040010337.g) from Passiflora edulis Sims, which has a coding sequence (CDS) of 1161 bp, encoding a protein consisting of 386 amino acid residues was cloned. The PeF3H protein contains a non-heme dioxygenase (DIOX-N superfamily) domain and a typical F3H protein functional domain (2OG-FeII-Oxy dioxygenase). Phylogenetic analysis revealed that the PeF3H protein shared high similarity with F3H proteins in Turnera subulata, Populus alba, and Populus tomentosa, with 88% identities of amino acid sequences. The PeF3H protein lacks a transmembrane structure, indicating it is likely to be expressed in the mitochondria. Additionally, 3D structure, protein and protein interaction, and KEGG pathway of PeF3H were anticipated based on homologous proteins. qRT-PCR analysis showed that PeF3H was highly expressed in leaves, followed by stems and roots. These studies have provided insights into the molecular mechanisms underlying flavonoid biosynthesis and predicted potential targets for genetic engineering to improve the nutritional and medicinal properties of passion fruit.
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