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Activity and Point Mutation G699V in PcoORP1 Confer Resistance to Oxathiapiprolin in Phytophthora colocasiae Field Isolates

文献类型： 外文期刊

作者： Wang, Zhixin 1 ; Ke, Qihan 2 ; Tao, Kezhu 2 ; Li, Qianqian 2 ; Xia, Yeqiang 3 ; Bao, Jiandong 4 ; Chen, Qinghe 1 ;

作者机构： 1.Hainan Univ, Hainan Yazhou Bay Seed Lab, Sanya Nanfan Res Inst, Sanya 572025, Peoples R China

2.Hainan Univ, Coll Plant Protect, Key Lab Green Prevent & Control Trop Plant Dis & P, Minist Educ, Haikou 570228, Peoples R China

3.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Peoples R China

关键词： oxathiapiprolin; Phytophthora colocasiae; resistance mechanism; point mutation; CRISPR; Cas9

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.895； 五年影响因子：6.048 ）

ISSN： 0021-8561

年卷期：

页码：

收录情况： SCI

摘要： The oxysterol-binding protein inhibitor oxathiapiprolin is a new fungicide for controlling oomycetes diseases. Besides, laboratory mutagenesis oxathiapiprolin-resistance among phytopathogenic oomycetes in the field remains unknown. Here, the sensitivity of 97 P. colocasiae isolates to oxathiapiprolin was examined that were collected between 2011 and 2016. We obtained a baseline sensitivity with a mean EC50 value of 5.2639 x 10-4 mu g mL-1. We showed that 6/32 isolates collected in Fujian Province from 2019 to 2020 were resistant to oxathiapiprolin without a significant fitness penalty on sporulation, vegetative growth, and virulence of the field isolates. The oxathiapiprolin resistance field isolates contained the point mutation glycine to valine at 699 in PcoORP1. The point mutation G699V was verified to confer resistance of P. colocasiae to oxathiapiprolin using the CRISPR/Cas9 system. The mutation G699V decreased the binding affinity between oxathiapiprolin and PcoORP1. These results will improve our understanding of the mechanism of P. colocasiae resistance to oxathiapiprolin under field conditions.

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