文献类型： 外文期刊

作者： Sun, Yujia ¹ ; Weng, Shaoping ¹ ; Xu, Binfu ⁴ ; Dong, Chuanfu ¹ ; He, Jianguo ¹ ;

作者机构： 1.Sun Yat sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China

2.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Guangdong, Peoples R China

3.Sun Yat sen Univ, Sch Marine Sci, Zhuhai 519000, Guangdong, Peoples R China

4.Fujian Acad Agr Sci, Inst Biotechnol, Fish Dis Unit, Fuzhou 350003, Peoples R China

5.Sun Yat sen Univ, Inst Aquat Econ Anim, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Peoples R China

6.Sun Yat sen Univ, Sch Life Sci, 135, Xingangxi Rd, Guangzhou 510275, Peoples R China

关键词： Pseudomonas plecoglossicida; Epinephelus coioides; iron-deficiency anemia; iron loss hypoxia spleen hemolysis

期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）

ISSN： 0044-8486

年卷期： 2023 年 563 卷

页码：

收录情况： SCI

摘要： Pseudomonas plecoglossicida is a causative agent of disease in aquaculture, leading to massive mortality in a variety of economically important cultured fish and resulting in great economic losses. Here we revealed that P. plecoglossicida infection caused a 62% probability of visceral white spot disease in orange-spotted grouper (Epinephelus coioides). After P. plecoglossicida challenge, the spleen mass increased significantly and the liver mass decreased significantly. Spleens had the highest bacterial load, at 1.38 x 107 colony forming units per gram. In addition, the number of blood red blood cells decreased. Moreover, diseased fish suffered from anemia and hypoxia. In addition, ferritin in the spleen was reduced, and there was a loss of iron in the fish. Furthermore, histological examination of the spleen showed that abundant red blood cells had infiltrated and that collagen fibers had been dissolved. In contrast, the survivors' spleens were rich in ferritin, having few red blood cell infiltration and collagen fibers that were intact. In summary, spleen collagen fibrinolysis, red blood cell infil-tration, and ferritin loss were demonstrated to cause hypoxia in the fish, which may be the reasons of death caused by P. plecoglossicida in orange-spotted grouper.