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Molecularly Imprinted Polymer-Based Electrochemical Sensor for In Situ Detection of Free Proline in Cucumber Leaves

文献类型： 外文期刊

作者： Yan, Lucheng 1 ; Luo, Bin 1 ; Wang, Cheng 1 ; Dong, Hongtu 1 ; Wang, Xiaodong 1 ; Hou, Peichen 1 ; Liu, Ke 1 ; Li, Aixue 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Res Ctr Intelligent Equipment, Beijing 100097, Peoples R China

2.China Agr Univ, Coll Informat & Elect Engn, Beijing 100083, Peoples R China

关键词： proline; screen-printed electrodes; molecularly imprinted polymers; electrochemical biosensors; in situ

期刊名称：CHEMELECTROCHEM （ 影响因子：4.0； 五年影响因子：4.0 ）

ISSN： 2196-0216

年卷期： 2024 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Proline is an important amino acid, which is crucial to plant growth and development. Accurate analysis of proline content is of great significance for understanding its physiological mechanism in plants. In this paper, according to the requirements for in-situ detection in plants in precision agriculture, an electrochemical molecular imprinted polymers (MIP) sensor for determining proline in living plants was developed. Polypyrrole (PPy) was used as the functional monomer. To improve the performance of the MIP sensor, Au nanoparticles (NPs) were electrodeposited on the screen-printed electrodes (SPEs) electrode. Thionine (Thi) was then electropolymerized on the SPEs to be used as an internal reference signal molecule. The MIP-based proline sensor has the widest detection range of 1x10-16 -0.01 M. And its detection limit is the lowest (9.18 aM) so far. It was also used for measuring free proline in the leaves of living cucumber seedlings under salt stress. The MIP-based proline sensor has an important prospect for detecting the physiological status of plants in situ and will play an important role in smart agriculture. An electrochemical MIP sensor for determining proline was developed. Polypyrrole was used as the functional monomers. Thionine was used as an internal reference signal. The MIP-based sensor has the lowest LOD and the widest detection range for proline so far. It is a very interesting method for the preparation of MIP-based electrochemical sensors for determining proline in situ in plants.image

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