The Role of BMP7 in the Proliferation of Hu Sheep Dermal Papilla Cells Is Influenced by DNA Methylation
文献类型： 外文期刊
作者： Lv, Xiaoyang 1 ; He, Mingliang 2 ; Wang, Shanhe 2 ; Zheng, Wenxin 4 ; Zhou, Hanlin 5 ; Mwacharo, Joram M. 6 ; Sun, Wei 1 ;
作者机构： 1.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou 225009, Peoples R China
2.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China
3.Yangzhou Univ, Int Joint Res Lab Univ Jiangsu Prov China Domest A, Yangzhou 225009, Peoples R China
4.Xinjiang Acad Anim Sci, Urumqi 830011, Peoples R China
5.Chinese Acad Trop Agr Sci, Zhanjiang Expt Stn, Zhanjiang 524000, Peoples R China
6.Int Ctr Agr Res Dry Areas, Addis Ababa, Ethiopia
关键词： Hu sheep; lamb skin; BMP7 gene; methylation; dermal papilla cell
期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.0 ）
ISSN： 2076-2615
年卷期： 2024 年 14 卷 11 期
页码：
收录情况： SCI
摘要： Previous studies have shown that the BMP7 gene is differentially expressed in Hu sheep lamb skin of different pattern types, and its expression level is significantly correlated with hair follicle indices of different pattern types, but the molecular mechanism of the differential expression of the BMP7 gene remains unclear. This study investigated the effect of DNA methylation on the transcriptional expression of BMP7. Firstly, we found that the mRNA expression of the BMP7 gene and the activity of the core promoter of the BMP7 gene were upregulated after 5-Aza-Deoxycytidine-induced demethylation treatment using qRT-PCR and double luciferase reporter assay. Then, we found that the proliferation of Hu sheep DPCs in vitro was promoted after 5-Aza-Deoxycytidine-induced demethylation treatment through qRT-PCR, CCK-8, and EdU assay, and that the overexpression of DNMT1 in DPCs induced the opposite effect. In addition, the results of the cell cycle assay reveal that the percentage of cells in the S phase was increased after 5-Aza-Deoxycytidine-induced demethylation treatment, and that the percentage of cells in the S phase was decreased after overexpression of DNMT1 in DPCs. This study indicated that the differential expression of the BMP7 gene in different patterns of Hu sheep lamb skin may be regulated by DNA methylation modification. In addition, DNA methylation can regulate the proliferation and cell cycle of DPCs in Hu sheep.
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