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Development of a Pedigreed Sorghum Mutant Library

文献类型： 外文期刊

作者： Chen, Junping 1 ; Zou, Guihua 1 ; Xin, Zhanguo 1 ;

作者机构： 1.ARS, Plant Stress & Germplasm Dev Unit, USDA, Lubbock, TX 79401 USA

2.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou, Zhejiang, Peoples R China

关键词： Sorghum bicolor; Sorghum; Ethylmethane sulfonate; EMS; Mutagenesis; Mutant library

期刊名称：SORGHUM: METHODS AND PROTOCOLS

ISSN： 1064-3745

年卷期： 2019 年 1931 卷

页码：

收录情况： SCI

摘要： Induced mutagenesis is a powerful approach to generate variations for elucidation of gene function and to create new traits for breeding. Here, we described a procedure to develop a pedigreed mutant library through chemical mutagenesis with ethylmethane sulfonate (EMS) treated seeds in sorghum and discussed its potential to generate new traits for sorghum improvement. Unlike random mutagenesis, a pedigreed mutant library, once properly established, can serve as a powerful resource to isolate and recover mutations of both agronomical and biological importance. With the development of affordable and high-throughput next-generation sequencing technologies, identification of causal mutations from a mutant library with a uniform genetic background becomes increasingly efficient and cost-effective. Fast causal gene discovery from mutant libraries combined with precise genome editing techniques will accelerate incorporation of new traits and revolutionize crop breeding.

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