Development of an immunochromatographic strip for rapid detection of recombinant rice EPSPS in transgenic crops
文献类型： 外文期刊
作者： Luo, Biao 1 ; Wang, Fang 2 ; Wang, Qiming 1 ; Li, Wanchang 2 ; Wang, Yan 2 ; Lin, Chaoyang 4 ; Wu, Wei 5 ; Fang, Ruiqiu 6 ; Rao, Liqun 1 ; Zhang, Xianwen 2 ;
作者机构： 1.Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Peoples R China
3.Yuelushan Lab, Changsha 410128, Peoples R China
4.Zhejiang Univ, Inst Insect Sci, Rural Dev Acad, Hangzhou 310063, Peoples R China
5.Shanghai YouLong Biotech Co Ltd, Shanghai 201114, Peoples R China
6.Zhejiang Acad Agr Sci, Inst Maize & Featured Upland Crops, Dongyang 322100, Peoples R China
7.Zhejiang Acad Agr Sci, Key Lab Traceabil Agr Genet Modified Organisms, Minist Agr & Rural Affairs, Hangzhou 310021, Peoples R China
关键词： 5-Enolpyruvyl-shikimate-3-phosphate synthase; (EPSPS); Immunochromatographic strip (ICS); Glyphosate-resistant crops; Rapid detection
期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：2.5； 五年影响因子：2.7 ）
ISSN： 0003-2697
年卷期： 2025 年 705 卷
页码：
收录情况： SCI
摘要： Glyphosate-resistant crops, developed using the EPSPS gene, are widely cultivated globally. Previous studies indicate that the TIPS-OsEPSPS gene provides glyphosate resistance in rice. In this research, the OsmEPSPS gene was utilized to develop glyphosate-resistant, maize, and soybean, and a rapid immunochromatographic strip (ICS) test was designed for specific detection of the OsmEPSPS protein. The ICS test employs a double-antibody sandwich format with two monoclonal antibodies: mAb #9, conjugated to colloidal gold for capture, and mAb #15, for detection at the test line. The ICS exhibited a limit of detection (LOD) of 0.031 mu g/mL within 10 min. Additionally, the ICS could detect a concentration of 0.817 mu g/mL within 1 min in both Eleusine indica and OsmEPSPS-overexpressing plants. The ICS is highly specific, sensitive, and stable, with an R2 value of 0.982. Notably, the ICS is capable of detecting EPSPS proteins with high homology to OsmEPSPS from Poaceae and other plants. In summary, this study developed a sensitive and specific test strip for OsmEPSPS detection, facilitating semiquantitative analysis on the basis of colorimetric response time and intensity. This ICS is not only suitable for the rapid identification of OsmEPSPS-overexpressing plants but also for phylogenetic analysis of EPSPS gene homology.
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