您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A Multiplex PCR System for the Screening of Genetically Modified (GM) Maize and the Detection of 29 GM Maize Events Based on Capillary Electrophoresis

文献类型： 外文期刊

作者： Yi, Hongmei 1 ; Liang, Ziyue 1 ; Ge, Jianrong 1 ; Zhang, Haibo 2 ; Liu, Fengze 3 ; Ren, Xuezhen 3 ; Ren, Jie 1 ; Wang, Haijie 1 ; Ren, Jiali 1 ; Ren, Xingxu 1 ; Zhang, Ying 2 ; Jin, Fang 3 ; Jin, Shiqiao 3 ; Zhao, Yikun 1 ; Wang, Fengge 1 ;

作者机构： 1.Beijing Acad Agr & Forest Sci BAAFS, Maize Res Ctr, Beijing Key Lab Maize DNA Fingerprinting & Mol Br, Beijing 100089, Peoples R China

2.Shaanxi Seed Adm Bur, Xian 710018, Peoples R China

3.Natl Agr Technol Extens & Serv Ctr, Beijing 100026, Peoples R China

关键词： genetically modified (GM) maize; multiplex polymerase chain reaction (PCR); capillary electrophoresis (CE)

期刊名称：AGRICULTURE-BASEL （ 影响因子：3.408； 五年影响因子：3.459 ）

ISSN：

年卷期： 2022 年 12 卷 3 期

页码：

收录情况： SCI

摘要： The detection of genetically modified (GM) maize events is an inevitable necessity under the strict regulatory systems of many countries. To screen for GM maize events, we developed a multiplex PCR system to specifically detect 29 GM maize events as well as the cauliflower mosaic virus 35S promoter, the Agrobacterium tumefaciens nos terminator, the Streptomyces viridochromogenes pat gene, and the endogenous zSSIIb maize reference gene. These targets were divided into five panels for screening and event-specific detection by multiplex (10-plex, 7-plex, 7-plex, 4-plex, and 5-plex) PCR. All amplification products were separated and visualized by fluorescence capillary electrophoresis (CE). By taking advantage of the high resolution, multiple fluorescence detection, and high sensitivity of CE, our system was able to identify all targets simultaneously with a limit of detection of 0.1%. The accurate identification of specific amplification peaks from different GM maize materials by CE confirmed the specificity of the system. To verify the practical applicability of this system, we analyzed 20 blind samples. We successfully identified five MON810, four TC1507, and three MIR162 samples. The detection of concomitant elements also verified the accuracy of this approach. Our system can, therefore, be used for the screening and detection of GM maize events. The system, which is easy to use, facilitates high-throughput detection with the help of a high-throughput platform and automated identification software. Multiplex PCR coupled with CE is, thus, very suitable for the detection of genetically modified organisms (GMOs) with a large number of detection targets. Additional multiplexed electrophoretic targets can be easily incorporated as well, thereby increasing the usefulness of this system as the number of GMO events continues to increase.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台