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CRISPR/Cas9-Mediated Disruption of lrp6a Leads to Abnormal Median Fin Development and Somitogenesis in Goldfish (Carassius auratus)

文献类型： 外文期刊

作者： Li, Huijuan 1 ; Zhang, Rong 1 ; Wang, Xiaowen 1 ; Liu, Lili 1 ; Yao, Zhigang 1 ; Zhu, Hua 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Fisheries Sci Inst, Beijing Key Lab Fishery Biotechnol, Beijing 100097, Peoples R China

关键词： CIRSPR/Cas9; lrp6a; Carassius auratus

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 15 期

页码：

收录情况： SCI

摘要： In this study, we demonstrated that lrp6a, a co-receptor in the Wnt signaling pathway, is essential for proper median fin formation and somitogenesis in goldfish. We analyzed the gene's sequence features and expression patterns in both wen-type and egg-type goldfish, uncovering distinct tissue-specific expression differences between the two varieties. To explore the functional role of lrp6a, we performed CRISPR/Cas9-mediated gene knockout using eight designed single-guide RNAs (sgRNAs), of which four showed effective targeting. Three high-efficiency sgRNAs were selected and co-injected into embryos to achieve complete gene disruption. Morphological assessments and X-ray microtomography (mu CT) imaging of the resulting mutants revealed various abnormalities, including defects in the dorsal, caudal, and anal fins, as well as skeletal deformities near the caudal peduncle. These results confirm that lrp6a plays a key role in median fin development and axial patterning, offering new insights into the genetic regulation of fin formation in teleost fish.

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