文献类型： 外文期刊

作者： Wang, Chaoqun ¹ ; Dor, Maoz ⁴ ; Kravchenko, Alexandra ⁴ ; Guber, Andrey ⁴ ; Dorodnikov, Maxim ⁵ ;

作者机构： 1.Univ Gottingen, Biogeochem Agroecosyst, D-37077 Gottingen, Germany

2.Univ British Columbia, Fac Land & Food Syst, Vancouver, BC V6T1Z4, Canada

3.Chinese Acad Trop Agr Sci, Inst Environm & Plant Protect, Key Lab Low Carbon Green Agr Trop Reg China, Minist Agr & Rural Affairs,Hainan Key Lab Trop Ec, Haikou 571101, Peoples R China

4.Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA

5.Univ Munster, Inst Landscape Ecol, D-48149 Munster, Germany

关键词： Anoxic conditions; beta-glucosidase; Phosphatase; Soil organic matter decomposition; Suppression by aeration; Time-lapse zymography

期刊名称：GEODERMA （ 影响因子：6.6； 五年影响因子：7.3 ）

ISSN： 0016-7061

年卷期： 2025 年 457 卷

页码：

收录情况： SCI

摘要： Land-use change and duration of agricultural practice cause shifts in the soil physical structure and porosity. These changes, in turn, may hamper soil aeration, restrict gas exchange, and therefore, cause establishment of anoxic conditions in upland soils. Such periodic fluctuations of aeration control the activity of microbial extracellular hydrolytic enzymes. Earlier studies revealed a clear suppression of key hydrolytic enzymes due to a short-term aeration (i.e., oxygen exposure) of established anoxic ecosystems such as rice paddies. However, it remains unclear whether the suppression also occurs in upland soils with periodic anoxicity. This key gap was addressed by measuring the kinetic parameters and in situ activities of hydrolytic beta-glucosidases and acid phosphatases under oxic and anoxic conditions in forest, grassland, and cropland mineral soil after 3-5 days of anoxic pre-incubation. Short-term (2 h) aeration suppressed beta-glucosidase and acid phosphatase activities by up to two times as compared with activities under anoxic conditions. The suppressive effects of aeration on the maximum reaction rate of enzymatic activity (Vmax) were more pronounced in the cropland (by 1.6-2.4 times) than in the forest (by 1.3-1.7 times) soil. Our findings indicate the importance of fluctuations in redox conditions in the soil organic matter transformation catalyzed by hydrolytic enzymes, particularly in scenarios where the frequency and/or severity of flooding events increase due to changes in land use and/or climatic conditions. The mechanism of the suppression of hydrolytic enzyme activities has been demonstrated to be sustainable in lowland and upland ecosystems, yet further studies are required to elucidate the biogeochemical basis for this phenomenon.