METTL3 Regulates the Inflammatory Response in CPB2 Toxin-Exposed IPEC-J2 Cells through the TLR2/NF-kappa B Signaling Pathway
文献类型： 外文期刊
作者： Zhang, Juanli 1 ; Yang, Jiaojiao 1 ; Gao, Xiaoli 1 ; Huang, Xiaoyu 1 ; Luo, Ruirui 2 ; Yang, Qiaoli 1 ; Yan, Zunqiang 1 ; Wang, Pengfei 1 ; Wang, Wei 3 ; Xie, Kaihui 1 ; Li, Jie 1 ; Zhang, Bo 2 ; Gun, Shuangbao 1 ;
作者机构： 1.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou 730070, Peoples R China
2.Gansu Acad Agr Sci, CAAS, Lanzhou 730070, Peoples R China
3.Northwest A&F Univ, Coll Anim Sci & Technol, Xian 712100, Peoples R China
4.Gansu Res Ctr Swine Prod Engn & Technol, Lanzhou 730070, Peoples R China
关键词： METTL3; CPB2 toxin; IPEC-J2; inflammatory response; TLR2/NF-kappa B
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 24 期
页码：
收录情况： SCI
摘要： Clostridium perfringens beta2 (CPB2) toxin is one of the main pathogenic toxins produced by Clostridium perfringens, which causes intestinal diseases in animals and humans. The N6-methyladenosine (m6A) modification is the most common reversible modification in eukaryotic disease processes. Methyltransferase-like 3 (METTL3) regulates immunity and inflammatory responses induced by the bacterial infections in animals. However, METTL3 & PRIME;s involvement in CPB2-treated intestinal porcine epithelial cell line-J2 (IPEC-J2) remains unclear. In the current study, we used methylated RNA immunoprecipitation-quantitative polymerase chain reaction, Western blotting and immunofluorescence assay to determine the role of METTL3 in CPB2-exposed IPEC-J2 cells. The findings revealed that m6A and METTL3 levels were increased in CPB2 treated IPEC-J2 cells. Functionally, METTL3 overexpression promoted the release of inflammatory factors, increased cytotoxicity, decreased cell viability and disrupted tight junctions between cells, while the knockdown of METTL3 reversed these results. Furthermore, METTL3 was involved in the inflammatory response of IPEC-J2 cells by activating the TLR2/NF-kappa B signaling pathway through regulating TLR2 m6A levels. In conclusion, METTL3 overexpression triggered the TLR2/NF-kappa B signaling pathway and promoted CPB2-induced inflammatory responses in IPEC-J2 cells. These findings may provide a new strategy for the prevention and treatment of diarrhea caused by Clostridium perfringens.
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