A missense mutation in WRKY32 converts its function from a positive regulator to a repressor of photomorphogenesis
文献类型： 外文期刊
作者： Zhou, Hua 1 ; Zhu, Wei 1 ; Wang, Xuncheng 2 ; Bian, Yeting 3 ; Jiang, Yan 1 ; Li, Jian 1 ; Wang, Lixia 4 ; Yin, Ping 4 ; Deng, Xing Wang 1 ; Xu, Dongqing 3 ;
作者机构： 1.Southern Univ Sci & Technol, Key Lab Mol Design Plant Cell Factory, Inst Plant & Food Sci, Sch Life Sci,Guangdong Higher Educ Inst,Dept Biol, Shenzhen 518055, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing Key Lab Environm Friendly Management Frui, Beijing 100097, Peoples R China
3.Nanjing Agr Univ, Coll Agr, Natl Ctr Soybean Improvement, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
4.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Hubei Hongshan Lab, Wuhan 430070, Peoples R China
5.Peking Univ, Peking Tsinghua Ctr Life Sci, Sch Adv Agr Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China
6.Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China
关键词： Arabidopsis; COP1; HY5; light signaling; photomorphogenesis; WRKY32
期刊名称：NEW PHYTOLOGIST （ 影响因子：10.323； 五年影响因子：10.768 ）
ISSN： 0028-646X
年卷期： 2022 年 235 卷 1 期
页码：
收录情况： SCI
摘要： CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) mediates various cellular and physiological processes in plants by targeting a large number of substrates for ubiquitination and degradation. In this study, we reveal that a substitution of Pro for Leu at amino acid position 409 in WRKY32 largely suppresses the short hypocotyls and expanded cotyledon phenotypes of cop1-6. WRKY32(P409L) promotes hypocotyl growth and inhibits the opening of cotyledons in Arabidopsis. Loss of WRKY32 function mutant seedlings display elongated hypocotyls, whereas overexpression of WRKY32 leads to shortened hypocotyls. WRKY32 directly associates with the promoter regions of HY5 to activate its transcription. COP1 interacts with and targets WRKY32 for ubiquitination and degradation in darkness. WRKY32(P409L) exhibits enhanced DNA binding ability and affects the expression of more genes compared with WRKY32 in Arabidopsis. Our results not only reveal the basic role for WRKY32 in promoting photomorphogenesis, but also provide insights into manipulating plant growth by engineering key components of light signaling.
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