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CaCl2-HCl electrolyzed water promotes glucosinolate metabolism in broccoli sprouts via calcium signalling

文献类型： 外文期刊

作者： Li, Cui 1 ; Song, Shuhui 2 ; He, Yanan 1 ; Han, Siyu 1 ; Liu, Haijie 1 ;

作者机构： 1.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Agrifood Proc & Nutr, Beijing 100097, Peoples R China

关键词： CaCl2-HCl electrolyzed water (CHEW); Broccoli sprouts; Glucosinolate metabolism; Calcium signal; Molecular mechanisms

期刊名称：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE （ 影响因子：6.6； 五年影响因子：6.6 ）

ISSN：

年卷期： 2023 年 10 卷 1 期

页码：

收录情况： SCI

摘要： Background Electrolyzed water, a green chemistry invention, is a novel disinfectant that has been widely used in the food field. Previous studies have found that slightly acidic electrolyzed water not only has a bactericidal effect but also promotes the accumulation of active substances in many types of sprouts. We developed a new type of electrolyzed water, CaCl2-HCl electrolyzed water (CHEW), which can effectively enhance the formation of glucosinolates and isothiocyanates in broccoli sprouts. The molecular mechanism by which CaCl2-HCl electrolyzed water promotes glucosinolate metabolism in broccoli sprouts during growth was investigated in this study. Results On the 4th day, the total glucosinolate content in broccoli sprouts reached 195.50 mu mol/g DW under CHEW treatment. On the 8th day, compared to treatment with tap water, the contents of three isothiocyanates (sulforaphane, erucin, and 4-isothiocyanato-1-butene) in broccoli sprouts under CHEW treatment were significantly increased by 45.93, 34.04 and 185.15%, respectively. The results of calcium distribution and qRT.PCR assessments indicated that CHEW treatment activated calcium signalling in broccoli sprouts and promoted the relative expression of glucosinolate biosynthesis-related genes through calcium sensors [calmodulin (CaM), calmodulin-like protein (CML), and calcium-dependent protein kinase (CDPK)] and the calcium-binding nuclear protein IQD1. Conclusion Our research provides new insights into the molecular mechanism of glucosinolate metabolism and a theoretical basis for cultivating high-quality broccoli sprouts.

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