文献类型： 外文期刊
作者： Jiang, Junfang 1 ; Cao, Yuhao 2 ; Shan, Huili 1 ; Wu, Jianliang 1 ; Song, Xuemei 2 ; Jiang, Yongqing 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet, Hangzhou, Peoples R China
2.Ningbo Univ, Zhejiang Key Lab Pathophysiol, Dept Biochem & Mol Biol, Sch Med, Ningbo, Peoples R China
关键词： Hu sheep; body size traits; genome wide association studies; SNPs; transcription activity; population verification
期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）
ISSN：
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Body size is an important indicator of growth and health in sheep. In the present study, we performed Genome-Wide Association Studies (GWAS) to detect significant single-nucleotide polymorphisms (SNPs) associated with Hu sheep's body size. After genotyping parental (G1) and offspring (G2) generation of the nucleus herd for meat production of Hu sheep and conducting GWAS on the body height, chest circumference, body length, tail length, and tail width of the two groups, 5 SNPs associated with body height and 4 SNPs correlated with chest circumference were identified at the chromosomal significance level. No SNPs were significantly correlated to body length, tail length, and width. Four out of the 9 SNPs were found to be located within the 4 genes. KITLG and CADM2 are considered as candidate functional genes related to body height; MCTP1 and COL4A6 are candidate functional genes related to chest circumference. The 9 SNPs found in GWAS were verified using the G3 generation of the nucleus herd for meat production. Nine products were amplified around the 9 sites, and 29 SNPs were found; 3 mutation sites, G > C mutation at 134 bp downstream of s554331, T > G mutation at 19 bp upstream of s26859.1, and A > G mutation at 81 bp downstream of s26859.1, were significantly correlated to the body height. Dual-luciferase reporter gene experiments showed that the 3 SNPs could significantly impact dual-luciferase and gene transcription activity.
- 相关文献
作者其他论文 更多>>
-
Novel polymorphism at ARHGAP24 gene and its association with growth traits in Hu sheep
作者：Shan, Huili;Huang, Xin;Jiang, Yongqing;He, Sangang;Jiang, Junfang;Guo, Liang yong;Zhang, Xiaowei
关键词：Rho GTPase activating protein 24; association analysis; single-nucleotide polymorphism; haplotype; Hu sheep
-
Rapid SNP genotyping detection method based on PCR-lateral flow dipstick detection technique
作者：Jiang, Junfang;Xiong, Pei;Huang, Xin;Zheng, Kaizhi;Jiang, Yongqing;He, Sangang;Shan, Huili;Zhang, Xiaowei
关键词：MC4R; SNP; Polymerase chain reaction (PCR); Lateral flow dipstick (LFD); Detection
-
High-concentrate diet decreases lamb fatty acid contents by regulating bile acid composition
作者：Zheng, Kaizhi;Cao, Yang;Jiang, Junfang;Huang, Xin;He, Sangang;Gao, Hui;Zhang, Xiaowei;Li, Kui;Guo, Liangyong;Yin, Yuyang;Li, Jinbing
关键词：High-concentrate diet; Metabolomics; Lamb; Bile acid; Fatty acid
-
The Effect of Mulberry Silage Supplementation on the Carcass Fatness and Long-Chain Fatty Acid Composition of Growing Lambs Compared with Traditional Corn Silage
作者：Cao, Yang;Zheng, Kaizhi;Wu, Jianliang;Lv, Zhiqiang;Huang, Xin;Jiang, Yongqing;Cao, Yang;Jiang, Junfang;Cao, Yang;Zhao, Xiaoou;Fang, Wenwen;Cao, Yang
关键词：lamb; mulberry; carcass fatness; long-chain fatty acid
-
Regulatory role of N6-Methyladenosine on skeletal muscle development in Hu sheep
作者：Jiang, Junfang;Huang, Xin;Zheng, Kaizhi;He, Sangang;Shan, Huili;Guo, Liangyong;Guo, Liangyong
关键词：Hu sheep; longissimus dorsi muscle; skeletal muscle development; m6A modification; MeRIP-seq
-
Proteome changes of dairy calves rumen epithelium from birth to postweaning
作者：Zheng, Kaizhi;Wu, Jianliang;Cao, Yang;Jiang, Yongqing;Huang, Xin;Jiang, Junfang;Ullah, Saif
关键词：proteome; dairy calves; rumen; epithelium; postnatal development
-
Proteomic and parallel reaction monitoring approaches to evaluate biomarkers of mutton tenderness
作者：Zheng, Kaizhi;Cao, Yang;Jiang, Junfang;Huang, Xin;Jiang, Yongqing;Wu, Jianliang;Yin, Yuyang;Guo, Liangyong;Li, Peng
关键词：Proteomics; PRM; Intensive fattening; Mutton; Tenderness; Biomarker
