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Validamycin affects the development and chitin metabolism in Spodoptera frugiperda by inhibiting trehalase activity

文献类型： 外文期刊

作者： Luo, Yu-Jia 1 ; Chen, Yan 1 ; Wang, Xiao-Jing 2 ; Wang, Si-Tong 1 ; Yang, Yuan-Yuan 1 ; Xu, Hong-Xing 3 ; Qu, Cheng 2 ; Wu, Yan 4 ; Li, Can 4 ; Wang, Shi-Gui 1 ; Tang, Bin 1 ; Wang, Su 2 ;

作者机构： 1.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 311121, Zhejiang, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Peoples R China

4.Guiyang Univ, Guizhou Prov Key Lab Rare Anim & Econ Insect Mount, Dept Biol & Engn Environm, Guiyang 550005, Peoples R China

关键词： Trehalase inhibitor; chitinase; glucose; fall armyworm; biopesticide

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：6.608； 五年影响因子：4.803 ）

ISSN： 0171-8177

年卷期： 2022 年 42 卷 6 期

页码：

收录情况： SCI

摘要： The fall armyworm (FAW) Spodoptera frugiperda is a major polyphagous pest that feeds mainly on food crops, such as corn and rice, posing a serious threat to the safety of food crops worldwide. Trehalase, can degrade trehalose and the resulting products participate in the synthesis of chitin, one of the main components of outer epidermis in insects. Therefore, trehalase inhibitors have been identified as novel agents for pest control. We report the insecticidal potential of a trehalase inhibitor validamycin against FAW. Validamycin (1 mu g/mu L) when injected in FAW using microinjection could significantly reduce trehalase activity 48 h later, especially, the activity of soluble trehalase, then the glucose content and glycogen content also decrease. In addition, the deaths of larvae in validamycin treated group at each instar stage were higher than those in the control group, moreover, validamycin treatment increased the pupal weight and prolonged pest development. Similarly, validamycin also significantly inhibited the chitinase activity and caused the accumulation of chitin in FAW. Results of qRT-PCR showed that the expression levels of two trehalose genes (SfTRE1 and SfTRE2), which encode key enzymes in chitin biosynthesis pathway, were significantly reduced, while the expression levels of fructose-6-phosphate transaminase, glucosamine-phosphate N-acetyltransferase and chitinase genes were significantly increased. It is obvious that validamycin has an influence on physiological metabolism and development of FAW, the results obtained will be conducive to the prevention and control of FAW in the future.

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