Fluorescent Probe Combined with Photoelectric Analysis Technology for Detection of Escherichia coli
文献类型： 外文期刊
作者： Cui, Qian 1 ; Zhong, Yongjie 1 ; Shang, Wenkai 1 ; Deng, Fuming 2 ; Wang, Buhua 1 ; Wu, Jiajia 3 ; Wang, Peng 3 ; Wan, Liudang 5 ; Wang, Keling 5 ; Fang, Lingchen 1 ; Dai, Rui 1 ; Zhang, Han 1 ; Ledesma-Amaro, Rodrigo 6 ; Zhang, Yunuo 1 ; Huang, Jiaomei 1 ;
作者机构： 1.Hainan Univ, State Key Lab Marine Resource Utilizat South China, Haikou 570228, Peoples R China
2.Chinese Acad Trop Agr Sci CATAS, Coconut Res Inst, Coconut Res Ctr, Haikou 570228, Peoples R China
3.Chinese Acad Sci, CAS Key Lab Marine Environm Corros, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Biofouling Inst Oceanol, Qingdao 266071, Peoples R China
5.ViewKr, Insititue Biotechnol, Haikou 570228, Peoples R China
6.Imperial Coll London, Imperial Coll Ctr Synthet Biol, Dept Bioengn, London SW7 2AZ, England
关键词： Escherichia coli; fluorescent probe; photoelectric analysis; microbial photoelectric detection system; Hainan coconut water
期刊名称：BIOSENSORS-BASEL （ 影响因子：5.4； 五年影响因子：5.7 ）
ISSN：
年卷期： 2023 年 13 卷 2 期
页码：
收录情况： SCI
摘要： Food safety is facing great challenges in preventing foodborne diseases caused by pathogenic pollution, especially in resource-limited areas. The rapid detection technique of microorganisms, such as immunological methods and molecular biological methods, plays a crucial key in timely bioanalysis and disease treatment strategies. However, it is difficult for these methods to simultaneously meet the criteria of simple operation, high specificity, and sensitivity, as well as low cost. Coconut water is known as the "water of life" in Hainan. It is a refreshing and nutritious beverage which is widely consumed due to its beneficial properties to health. Coconut water processing is an important pillar industry in Hainan. The detection of pathogenic microorganisms, such as Escherichia coli, in coconut water has become an important factor which has restricted the upgrading and development of this industry. Based on the needs of industrial development, we developed a microbial photoelectric detection system which was composed of a fluorescent probe detection reagent and a photoelectric sensor detection device. This system combined microbial enzyme targets, selective fluorescent substrate metabolism characteristics, and a photoelectric sensor signal transduction mechanism, which produce a strong signal with a high signal-to-noise ratio. The microbial detection system developed here has a simple structure, simple and convenient operation, short detecting time (>= 2 h), and high sensitivity (1 CFU/mL). This system may also enable early warning and monitoring programs for other pathogenic microorganisms in order to promote the overall competitiveness of the Hainan coconut water industry.
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