文献类型： 外文期刊

作者： Zhu, Keyu ¹ ; Li, Miaomiao ¹ ; Wu, Haibin ³ ; Zhang, Deyun ⁴ ; Dong, Lingli ¹ ; Wu, Qiuhong ¹ ; Chen, Yongxing ¹ ; Xie, Jingzhong ¹ ; Lu, Ping ¹ ; Guo, Guanghao ¹ ; Zhang, Huaizhi ¹ ; Zhang, Panpan ¹ ; Li, Beibei ¹ ; Li, Wenling ¹ ; Dong, Lei ¹ ; Wang, Qifei ⁵ ; Zhu, Jinghuan ⁵ ; Hu, Wenli ⁶ ; Guo, Liqiao ⁶ ; Wang, Rongge ⁶ ; Yuan, Chengguo ⁶ ; Li, Hongjie ⁷ ; Liu, Zhiyong ¹ ; Hua, Wei ⁵ ;

作者机构： 1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.Acad Natl Food & Strateg Reserves Adm, Beijing 100037, Peoples R China

4.Chaozhou Hybribio Biochem Ltd, Chaozhou 521011, Guangdong, Peoples R China

5.Zhejiang Acad Agr Sci, Hangzhou 310021, Peoples R China

6.Hebei Gaoyi Stock Seed Farm, Gaoyi 051330, Hebei, Peoples R China

7.Chinese Acad Agr Sci, Inst Crop Sci, Natl Engn Lab Crop Mol Breeding, Beijing 100081, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.574； 五年影响因子：5.662 ）

ISSN： 0040-5752

年卷期： 2022 年 135 卷 4 期

页码：

收录情况： SCI

摘要： Key message Powdery mildew resistance gene MlWE74, originated from wild emmer wheat accession G-748-M, was mapped in an NBS-LRR gene cluster of chromosome 2BS. Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a globally devastating disease. Wild emmer wheat (Triticum turgidum var. dicoccoides) is a valuable genetic resource for improving disease resistance in common wheat. A powdery mildew resistance gene was transferred to hexaploid wheat line WE74 from wild emmer accession G-748-M. Genetic analysis revealed that the powdery mildew resistance in WE74 is controlled by a single dominant gene, herein temporarily designated MlWE74. Bulked segregant analysis (BSA) and molecular mapping delimited MlWE74 to the terminal region of chromosome 2BS flanking by markers WGGBD412 and WGGBH346 within a genetic interval of 0.25 cM and corresponding to 799.9 kb genomic region in the Zavitan reference sequence. Sequence annotation revealed two phosphoglycerate mutase-like genes, an alpha/beta-hydrolases gene, and five NBS-LRR disease resistance genes that could serve as candidates for map-based cloning of MlWE74. The geographical location analysis indicated that MlWE74 is mainly distributed in Rosh Pinna and Amirim regions, in the northern part of Israel, where environmental conditions are favorable to the occurrence of powdery mildew. Moreover, the co-segregated marker WGGBD425 is helpful in marker-assisted transfer of MlWE74 into elite cultivars.