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The Thioredoxin System in Edwardsiella piscicida Contributes to Oxidative Stress Tolerance, Motility, and Virulence

文献类型： 外文期刊

作者： He, Jiaojiao 1 ; Liu, Su 1 ; Fang, Qingjian 2 ; Gu, Hanjie 2 ; Hu, Yonghua 2 ;

作者机构： 1.Hainan Univ, Sch Life Sci, Haikou 570228, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China

3.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China

4.Hainan Prov Key Lab Funct Components Res & Utiliza, Haikou 571101, Peoples R China

5.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China

关键词： Edwardsiella piscicida; thioredoxin system; oxidative stress; motility; virulence

期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 11 卷 4 期

页码：

收录情况： SCI

摘要： Edwardsiella piscicida is an important fish pathogen that causes substantial economic losses. In order to understand its pathogenic mechanism, additional new virulence factors need to be identified. The bacterial thioredoxin system is a major disulfide reductase system, but its function is largely unknown in E. piscicida. In this study, we investigated the roles of the thioredoxin system in E. piscicida (named TrxB(Ep), TrxA(Ep), and TrxC(Ep), respectively) by constructing a correspondingly markerless in-frame mutant strain: Delta trxB, Delta trxA, and Delta trxC, respectively. We found that (i) TrxB(Ep) is confirmed as an intracellular protein, which is different from the prediction made by the Protter illustration; (ii) compared to the wild-type strain, Delta trxB exhibits resistance against H2O2 stress but high sensitivity to thiol-specific diamide stress, while Delta trxA and Delta trxC are moderately sensitive to both H2O2 and diamide conditions; (iii) the deletions of trxB(Ep), trxA(Ep), and trxC(Ep) damage E. piscicida's flagella formation and motility, and trxB(Ep) plays a decisive role; (iv) deletions of trxB(Ep), trxA(Ep), and trxC(Ep) substantially abate bacterial resistance against host serum, especially trxB(Ep) deletion; (v) trxA(Ep) and trxC(Ep), but not trxB(Ep), are involved in bacterial survival and replication in phagocytes; (vi) the thioredoxin system participates in bacterial dissemination in host immune tissues. These findings indicate that the thioredoxin system of E. piscicida plays an important role in stress resistance and virulence, which provides insight into the pathogenic mechanism of E. piscicida.

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