文献类型： 外文期刊

作者： Qing, CM ¹ ; Fan, L ² ; Lei, Y ² ; Bouchez, D ² ; Tourneur, C ² ; Yan, L ² ; Robaglia, C ² ;

作者机构： 1.Beijing Vegetable Res Ctr, Beijing 100089, Peoples R China

2.Beijing Vegetable Res Ctr, Beijing 100089, Peoples R China; INRA, Biol Cellulaire Lab, F-78026 Versailles, France

关键词： Agrobacterium tumefaciens; genetic transformation; herbicide resistance; crucifers; Brassica rapa; in planta transformation; in vivo selection

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2000 年 6 卷 1 期

页码：

收录情况： SCI

摘要： Transgenic pakchoi (Brassica rapa L. ssp. chinensis) plants were obtained in the progeny of plants infiltrated by an Agrobacterium tumefaciens strain carrying a gene for resistance to the herbicide phosphinotricin (Basta). Genetic analysis demonstrates the transmission of the herbicide resistant trait to the progeny. Molecular analyses show that the transgene was inserted in the plant genome and expressed. This work demonstrates that the infiltration transformation method originally devised for Arabidopsis thaliana can be adapted for other crucifer species and opens up the possibility of genetic engineering of pakchoi, an important vegetable plant.