文献类型： 外文期刊

作者： Zhang, FL ¹ ; Takahata, Y ² ; Watanabe, M ² ; Xu, JB ² ;

作者机构： 1.Iwate Univ, Fac Agr, Morioka, Iwate 0208550, Japan

2.Iwate Univ, Fac Agr, Morioka, Iwate 0208550, Japan; Beijing Vegetable Res Ctr, Beijing 100089, Peoples R China

关键词： Chinese cabbage (Brassica campestris L.; ssp pekinensis); genetic transformation; Agrobacterium tumefaciens; cotyledonary explant

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2000 年 19 卷 6 期

页码：

收录情况： SCI

摘要： A procedure for producing transgenic Chinese cabbage plants by inoculating cotyledonary explants with Agrobacterium tumefaciens strain EHA101 carrying a binary vector pIG121Hm, which contains kanamycin-resistance and hygromycin-resistance genes and the GUS reporter gene, is described. Infection was most effective (highest infection frequency) when explants were infected with Agrobacterium for 15 min and co-cultivated for 3 days in co-cultivation medium at pH 5.2 supplemented with 10 mg/l acetosyringone. Transgenic plants of all three cultivars used were obtained with frequencies of 1.6-2.7% when the explants were regenerated in shoot regeneration medium solidified with 1.6% agar. A histochemical GUS assay and PCR and Southern blot analyses confirmed that transformation had occurred. Genetic analysis of T1 progeny showed that the transgenes were inherited in a Mendelian fashion.