文献类型： 外文期刊

作者： Levi, A. ¹ ; Thomas, C. E. ² ; Trebitsh, T. ² ; Salman, A. ² ; King, J. ² ; Karalius, J. ² ; Newman, M. ² ; Reddy, O. U. ¹ ;

作者机构： 1.USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA

2.USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA; Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel; Seminis Vegetable Seeds, Woodland, CA 95695 USA; USDA ARS, Plant Genet Resources & Conservat Unit, Griffin, GA 30223 USA; W Virginia State Univ, Dept Biol, Institute, WV 25112 USA; Natl Engn Res Ctr Vegetables, Beijing 100089, Peoples R China; Syngenta Seeds, Woodland, CA 95695 USA

关键词： Citrullus lanatus; Citrullus colocynthis; genetic mapping; DNA markers; vegetable breeding

期刊名称：JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE （ 影响因子：1.144； 五年影响因子：1.617 ）

ISSN： 0003-1062

年卷期： 2006 年 131 卷 3 期

页码：

收录情况： SCI

摘要： Seventy-one amplified fragment length polymorphism (AFLP), 93 sequence related amplified polymorphism (SRAP), and 14 simple sequence repeat (SSR) markers were used to extend an initial genetic linkage map for watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai]. The initial map was based on 151 randomly amplified polymorphic DNA (RAPD) and 30 and inter-simple sequence repeat (ISSR) markers. A testcross population previously used for mapping of RAPD and ISSR markers was used in this study: (plant accession Griffin 14113 [C. lanatus var. citroide (L.H. Bailey) Mansf.] x the watermelon cultivar New Hampshire Midget (C. lanatus var. lanatus)) x PI 386015 [C. colocynthis (L.) Schrad.]. The linkage map contains 360 DNA markers distributed on 19 linkage groups, and covers a genetic distance of 1976 cM with an average distance of 5.8 cM between two markers. A genomic DNA clone representing 1-amino-cyclopropane-1-carboxylic acid (ACC-) synthase gene, involved in ethylene biosynthesis, was also mapped. As in previous mapping studies for watermelon, a large number of AFLP and SRAP markers were skewed away from the 1:1 segregation ratio, and had to be excluded from the final mapping analysis. The stringent mapping criteria (JoinMap 3.0 mapping program) produced linkage groups with marker order consistent with those reported in previous mapping study for watermelon.