文献类型： 外文期刊

作者： Zhang, F. L. ¹ ; Wang, M. ¹ ; Liu, X. C. ¹ ; Zhao, X. Y. ¹ ; Yang, J. P. ² ;

作者机构： 1.Natl Engn Res Ctr Vegetables, Beijing 100089, Peoples R China

2.Shandong Agr Univ, Hort Coll, Tai An 271018, Peoples R China

关键词： Brassica rapa ssp pekinensis; Turnip mosaic virus resistance; doubled haploid; genetic mapping

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN： 0179-9541

年卷期： 2008 年 127 卷 1 期

页码：

收录情况： SCI

摘要： Turnip mosaic virus ( TuMV) as the major virus infecting Brassica crops, often cause severe yield and quality losses in Chinese cabbage production in China. In this study, quantitative trait loci ( QTL) analysis for TuMV resistance was conducted with a population of 100 doubled-haploid lines derived from the F-1 between 91-112 ( resistant) and T12-19 ( susceptible) through microspore culture. A total of 376 molecular markers including 235 amplified fragment length polymorphisms, 129 random amplified polymorphic DNAs, 10 simple sequence repeats, one SCAR and one morphological marker were employed to construct a linkage map with 10 linkage groups covering 809.1 cM with an average distance of 2.2 cM between loci. Resistance was assessed by artificial inoculation at the seedling stage and at the adult stage in field conditions, respectively. Four QTLs controlling TuMV resistance were identified with JoinMap QTL 4.0 and interval mapping method. Two QTLs ( Tu1, Tu2) were associated with resistance at the seedling stage, each accounting for 58.2% and 14.7% of the phenotypic variation, respectively. Two QTLs ( Tu3, Tu4) were found corresponding to the disease resistance at the adult plant stage, explaining 48.5% and 32.0% of the phenotypic variation, respectively. Marker-assisted selection for these major QTLs involved in TuMV resistance could be useful in Chinese-cabbage breeding programmes.