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High human GLUT1, GLUT2, and GLUT3 expression in Schizosaccharomyces pombe

文献类型： 外文期刊

作者： Yang, Yuxin 1 ; Hu, Zongli 1 ; Liu, Zhizhao 1 ; Wang, Yi 1 ; Chen, Xuqing 1 ; Chen, Guoping 1 ;

作者机构： 1.Chongqing Univ, Coll Bioengn, Chongqing 400044, Peoples R China

2.Beijing Res Ctr Agrobiotechnol, Beijing 100089, Peoples R China

关键词： GLUT; Schizosaccharomyces pombe; membrane protein expression; GST-affinity chromatography

期刊名称：BIOCHEMISTRY-MOSCOW （ 影响因子：2.487； 五年影响因子：2.57 ）

ISSN： 0006-2979

年卷期： 2009 年 74 卷 1 期

页码：

收录情况： SCI

摘要： In this study, three subfamily members of the human 12-transmembrane-domain cell-surface receptors GLUT1, GLUT2, and GLUT3 were heterologously expressed in the fission yeast Schizosaccharomyces pombe utilizing GST-GLUT fusion proteins. These fusion proteins were driven by the full-length nmt1 promoter (Pnmt1) derived from S. pombe. The transcription levels of the GST-GLUT fusion proteins were very high upon induction by removing thiamine from the media. One-step purification of the recombinant fusion proteins was achieved by GST-affinity chromatography. Approximately 300 A mu g of highly purified fusion protein were obtained from 3 g of wet cell paste (1 liter of cell culture), indicating that human membrane proteins can be efficiently expressed and purified in the fission yeast. With its available extensive genetic information and ease of genetic manipulation, the fission yeast is potentially a highly efficient host to express eukaryotic membrane proteins.

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