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Populus euphratica HSF binds the promoter of WRKY1 to enhance salt tolerance

文献类型： 外文期刊

作者： Shen, Zedan 1 ; Yao, Jun 1 ; Sun, Jian 2 ; Chang, Liwei 1 ; Wang, Shaojie 1 ; Ding, Mingquan 3 ; Qian, Zeyong 1 ; Zhang, 1 ;

作者机构： 1.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China

2.Jiangsu Normal Univ, Sch Life Sci, Inst Integrat Plant Biol, Xuzhou 221116, Jiangsu, Peoples R China

3.Zhejiang Agr & Forestry Univ, Coll Agr & Food Sci, Hangzhou 317300, Zhejiang, Peoples R China

4.Natl Engn Res Ctr Informat Technol Agr, Beijing 100097, Peoples R China

关键词： Heat-shock transcription factor; Heat shock element; PeWRKY1; PeWRKY1 promoter; Gene silencing; NaCl

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2015 年 235 卷

页码：

收录情况： SCI

摘要： Poplar species increase expressions of transcription factors to deal with salt environments. We assessed the salt-induced transcriptional responses of heat-shock transcription factor (HSF) and WRKY1 in Populus euphratica, and their roles in salt tolerance. High NaCl (200 mM) induced PeHSF and PeWRKY1 expressions in P. euphratica, with a rapid rise in roots than in leaves. Moreover, the salt-elicited PeHSF reached its peak level 6 h earlier than PeWRKY1 in leaves. PeWRKY1 was down-regulated in salinized P. euphratica when PeHSF was silenced by tobacco rattle virus-based gene silencing. Subcellular assays in onion epidermal cells and Arabidopsis protoplasts revealed that PeHSF and PeWRKY1 were restricted to the nucleus. Transgenic tobacco plants overexpressing PeWRKY1 showed improved salt tolerance in terms of survival rate, root growth, photosynthesis, and ion fluxes. We further isolated an 1182-bp promoter fragment upstream of the translational start of PeWRKY1 from P. euphratica. Promoter sequence analysis revealed that PeWRKY1 harbours four tandem repeats of heat shock element (HSE) in the upstream regulatory region. Yeast one-hybrid assay showed that PeHSF directly binds the cis-acting HSE. To determine whether the HSE cluster was important for salt-induced PeWRKY1 expression, the promoter-reporter construct PeWRKY1-pro::GUS was transferred to tobacco plants. beta-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. Therefore, we conclude that salinity increased PeHSF transcription in P. euphratica, and that PeHSF binds the cis-acting HSE of the PeWRKY1 promoter, thus activating PeWRKY1 expression. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

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