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Comparative Transcriptome Analysis of Cultivated and Wild Watermelon during Fruit Development

文献类型： 外文期刊

作者： Guo, Shaogui 1 ; Sun, Honghe 2 ; Zhang, Haiying 2 ; Liu, Jingan 2 ; Ren, Yi 2 ; Gong, Guoyi 2 ; Jiao, Chen 3 ; Zheng, Yi; 1 ;

作者机构： 1.China Agr Univ, Coll Agron & Biotechnol, Dept Vegetable Sci, Beijing Key Lab Growth & Dev Regulat Protected Ve, Beijing 100094, Peoples R China

2.Natl Engn Res Ctr Vegetables, Key Lab Biol & Genet Improvement Hort Crops North, Beijing, Peoples R China

3.Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA

4.USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 6 期

页码：

收录情况： SCI

摘要： Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an important vegetable crop world-wide. Watermelon fruit quality is a complex trait determined by various factors such as sugar content, flesh color and flesh texture. Fruit quality and developmental process of cultivated and wild watermelon are highly different. To systematically understand the molecular basis of these differences, we compared transcriptome profiles of fruit tissues of cultivated watermelon 97103 and wild watermelon PI296341-FR. We identified 2,452, 826 and 322 differentially expressed genes in cultivated flesh, cultivated mesocarp and wild flesh, respectively, during fruit development. Gene ontology enrichment analysis of these genes indicated that biological processes and metabolic pathways related to fruit quality such as sweetness and flavor were significantly changed only in the flesh of 97103 during fruit development, while those related to abiotic stress response were changed mainly in the flesh of PI296341-FR. Our comparative transcriptome profiling analysis identified critical genes potentially involved in controlling fruit quality traits including alpha-galactosidase, invertase, UDP-galactose/glucose pyrophosphorylase and sugar transporter genes involved in the determination of fruit sugar content, phytoene synthase, beta-carotene hydroxylase, 9-cis-epoxycarotenoid dioxygenase and carotenoid cleavage dioxygenase genes involved in carotenoid metabolism, and 4-coumarate: coenzyme A ligase, cellulose synthase, pectinesterase, pectinesterase inhibitor, polygalacturonase inhibitor and alpha-mannosidase genes involved in the regulation of flesh texture. In addition, we found that genes in the ethylene biosynthesis and signaling pathway including ACC oxidase, ethylene receptor and ethylene responsive factor showed highly ripening-associated expression patterns, indicating a possible role of ethylene in fruit development and ripening of watermelon, a non-climacteric fruit. Our analysis provides novel insights into watermelon fruit quality and ripening biology. Furthermore, the comparative expression profile data we developed provides a valuable resource to accelerate functional studies in watermelon and facilitate watermelon crop improvement.

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