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Tissue-specific accumulation and subcellular localization of chalcone isomerase (CHI) in grapevine

文献类型： 外文期刊

作者： Wang, Huiling 1 ; Wang, Wei 2 ; Zhan, Jicheng 4 ; Huang, Weidong 4 ; Xu, Haiying 1 ;

作者机构： 1.Beijing Acad Forestry & Pomol Sci, Beijing 100097, Peoples R China

2.Chinese Acad Forestry, Inst Forestry, Key Lab Silviculture State Forestry Adm, Beijing 100091, Peoples R China

3.Minist Agr, Key Lab Urban Agr North China, Shanghai, Peoples R China

4.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

5.Beijing Engn Res Ctr Deciduous Fruit Trees, Beijing 100097, Peoples R China

关键词： Grapevine; Chalcone isomerase; Flavonoids; Immunolocalization

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2019 年 137 卷 1 期

页码：

收录情况： SCI

摘要： Flavonoids are widely distributed secondary metabolic products with many biological functions in plants. Further elucidation of the accumulation and localization patterns of its biosynthesis enzymes will broaden our understanding of flavonoids biosynthesis and regulation. Chalcone isomerase (CHI, EC 5.5.1.6) is an early-step enzyme in the flavonoids biosynthesis pathway. In this study, using an antibody specifically developed against grapevine CHI enzyme, we found that the accumulation of CHI protein exhibited temporal and spatial specificity. In grape berries, CHI was investigated mainly in the outer hypodermis cells of exocarp tissues, in the vascular bundles of mesocarp; and in the integument and the cells around the raphe of seeds. At the subcellular level, CHI was visualized in the cytoplasm, nucleus, and plastids (chloroplasts) of the exocarp cells, while only in the cytoplasm of mesocarp vascular bundle cells. In grapevine vegetable organs, the leaf mesophyll and phloem of leaf veins, the pith ray and primary phloem of stems, the primary phloem and endoderm of roots, and the young leaves, leaf primordium, and the growth point of leaf buds were CHI signal-positive. In these tissue cells, CHI was primarily observed in the cytoplasm, cell wall, and nucleus. The distinct localization patterns of CHI suggested the complexity of flavonoids biosynthesis in grapevine. Key MessageThe distribution of CHI protein exhibited temporal and spatial specificity. Different subcellular localization patterns were observed in grape berries and grapevine vegetable tissues.

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