Bulked Segregant Analysis Coupled with Whole-Genome Sequencing (BSA-Seq) Mapping Identifies a Novel pi21 Haplotype Conferring Basal Resistance to Rice Blast Disease
文献类型： 外文期刊
作者： Liang, Tingmin 1 ; Chi, Wenchao 1 ; Huang, Likun 3 ; Qu, Mengyu 1 ; Zhang, Shubiao 3 ; Chen, Zi-Qiang 2 ; Chen, Zai-Ji 1 ;
作者机构： 1.Minjiang Univ, Inst Oceanog, Marine & Agr Biotechnol Lab, Fuzhou 350108, Peoples R China
2.Fujian Acad Agr Sci, Biotechnol Res Inst, Fuzhou 350003, Peoples R China
3.Fujian Agr & Forestry Univ, Coll Agr, Fuzhou 350002, Peoples R China
4.Fujian Agr & Forestry Univ, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Peoples R China
关键词： rice; blast disease; partial resistance; pi21; haplotype
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2020 年 21 卷 6 期
页码：
收录情况： SCI
摘要： Basal or partial resistance has been considered race-non-specific and broad-spectrum. Therefore, the identification of genes or quantitative trait loci (QTLs) conferring basal resistance and germplasm containing them is of significance in breeding crops with durable resistance. In this study, we performed a bulked segregant analysis coupled with whole-genome sequencing (BSA-seq) to identify QTLs controlling basal resistance to blast disease in an F-2 population derived from two rice varieties, 02428 and LiXinGeng (LXG), which differ significantly in basal resistance to rice blast. Four candidate QTLs, qBBR-4, qBBR-7, qBBR-8, and qBBR-11, were mapped on chromosomes 4, 7, 8, and 11, respectively. Allelic and genotypic association analyses identified a novel haplotype of the durable blast resistance gene pi21 carrying double deletions of 30 bp and 33 bp in 02428 (pi21-2428) as a candidate gene of qBBR-4. We further assessed haplotypes of Pi21 in 325 rice accessions, and identified 11 haplotypes among the accessions, of which eight were novel types. While the resistant pi21 gene was found only in japonica before, three Chinese indica varieties, ShuHui881, Yong4, and ZhengDa4Hao, were detected carrying the resistant pi21-2428 allele. The pi21-2428 allele and pi21-2428-containing rice germplasm, thus, provide valuable resources for breeding rice varieties, especially indica rice varieties, with durable resistance to blast disease. Our results also lay the foundation for further identification and functional characterization of the other three QTLs to better understand the molecular mechanisms underlying rice basal resistance to blast disease.
- 相关文献
作者其他论文 更多>>
-
Editing the RR-TZF Gene Subfamily in Rice Uncovers Potential Risks of CRISPR/Cas9 for Targeted Genetic Modification
作者：Zhou, Shufen;Lu, Xiaozhuan;Wu, Weiren;Zhou, Shufen;Tian, Dagang;Liu, Huaqing;Zhang, Di;Chen, Rui;Yang, Shaohua;Wang, Feng
关键词：CRISPR/Cas9;
RR-TZF gene subfamily; rice (Oryza sativa L.); off-target effects; inheritable mutagenicity -
Effects of soy flour formulation and pretreatment on the properties of gluten-free cookies: A comprehensive study from flour, dough, to baked products
作者：Zhang, Yiqin;Zhou, Jianjun;Tian, Wenfei;Gui, Yijie;Li, Yonghui;Tian, Wenfei;Gui, Yijie
关键词：Gluten-free cookies; Soy flour; Modification; Mixing; Texture
-
Quasi-Targeted Metabonomics Reveals Metabolites Associated with Antioxidant Activity of Mesona chinensis Benth Cultivar Xiaoye
作者：Niu, Yuqing;Zheng, Meixia;Zhu, Yanming;Chen, Hong;Zhu, Yujing;Su, Hailan;Tian, Dagang
关键词：
M. chinensis Benth; Xiaoye variety; secondary metabolites; antioxidant activity; UHPLC-MS -
Protein Phosphatases MoPtc5, MoPtc1, and MoPtc2 Contribute to the Vegetative Growth, Stress Adaptation, and Virulence of Magnaporthe oryzae
作者：Biregeya, Jules;Otieno, Frankline Jagero;Mabeche, Anjago Wilfred;Felix, Abah;Aimable, Nsanzinshuti;Abubakar, Yakubu Saddeeq;Aron, Osakina;Lu, Guodong;Wang, Zonghua;Tang, Wei;Biregeya, Jules;Hong, Yonghe;Chen, Meilian;Wang, Zonghua
关键词：
Magnaporthe oryzae ; synergistic; stress tolerance; protein phosphatases; pathogenesis -
The OsZHD1 and OsZHD2, Two Zinc Finger Homeobox Transcription Factor, Redundantly Control Grain Size by Influencing Cell Proliferation in Rice
作者：Guo, Mingliang;She, Zeyuan;Guo, Mingliang;Tian, Dagang;Zheng, Chun;Shi, Chao;Lu, Xiaozhuan;Jiang, Shuyu;Qin, Yuan
关键词：Rice; Zinc finger-homeodomain;
OsZHD1 ;OsZHD2 ; Grain size; Cell proliferation -
Enhanced and Integrated Crop Management Improved Hard Red Winter Wheat Grain Yield and End-Use Characteristics
作者：Gui, Yijie;Gui, Yijie;Chen, Gengjun;Tian, Wenfei;Li, Yonghui;Chen, Gengjun;Tian, Wenfei;Jaenisch, Brent R.;Lollato, Romulo P.
关键词：agronomic management; bread-making; end-use quality; enhanced fertility; environment; hard red winter wheat; red winter wheat
-
Development of Rc-targeted molecular marker for breeding anthocyanin-rich red rice
作者：Zhang, Shuijin;Lu, Xiaozhuan;Zhou, Peng;Tian, Dagang;Chen, Jianmin;Chen, Chunhua
关键词：Rice;
Rc ; Red pericarp; MAS; Anthocyanin
