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Identification and Characterization of microRNAs in the Developing Seed of Linseed Flax (Linum usitatissimum L.)

文献类型： 外文期刊

作者： Zhang, Tianbao 1 ; Li, Zhen 1 ; Song, Xiaxia 1 ; Han, Lida 1 ; Wang, Limin 2 ; Zhang, Jianping 2 ; Long, Yan 1 ; Pei, Xin 1 ;

作者机构： 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

2.Gansu Acad Agr Sci, Crop Inst, Lanzhou 730070, Peoples R China

关键词： microRNA; miRNA156; seed development; fatty acid synthesis; linseed flax

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 8 期

页码：

收录情况： SCI

摘要： Seed development plays an important role during the life cycle of plants. Linseed flax is an oil crop and the seed is a key organ for fatty acids synthesis and storage. So it is important to understand the molecular mechanism of fatty acid biosynthesis during seed development. In this study, four small RNA libraries from early seeds at 5, 10, 20 and 30 days after flowering (DAF) were constructed and used for high-throughput sequencing to identify microRNAs (miRNAs). A total of 235 miRNAs including 114 known conserved miRNAs and 121 novel miRNAs were identified. The expression patterns of these miRNAs in the four libraries were investigated by bioinformatics and quantitative real-time polymerase chain reaction (qPCR) analysis. It was found that several miRNAs, including Lus-miRNA156a was significantly correlated with seed development process. In order to confirm the actual biological function of Lus-miRNA156a, over-expression vector was constructed and transformed to Arabidopsis. The phenotypes of homozygous transgenic lines showed decreasing of oil content and most of the fatty acid content in seeds as well as late flowering time. The results provided a clue that miRNA156a participating the fatty acid biosynthesis pathway and the detailed molecular mechanism of how it regulates the pathway needs to be further investigated.

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