文献类型： 外文期刊
作者： Jing, Xiaoshu 1 ; Yao, Jun 1 ; Ma, Xujun 3 ; Zhang, Yanli 1 ; Sun, Yuanling 1 ; Xiang, Min 4 ; Hou, Peichen 5 ; Li, Niya; 1 ;
作者机构： 1.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China
2.Shandong Univ, Sch Life Sci, State Key Lab Microbial Technol, Helmholtz Inst Biotechnol, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Urat Desert Grassland Res Stn, Lanzhou 730000, Peoples R China
4.Hainan Normal Univ, Coll Life Sci, Dept Biol, Haikou 571158, Hainan, Peoples R China
5.Beijing Acad Agr & Forestry Sci, Beijing Res Ctr Intelligent Equipment Agr, Beijing 100097, Peoples R China
关键词： thioredoxin; Kandelia candel; mannitol; drought; water retaining capacity; soluble sugar; H2O2; stomatal aperture; abscisic acid; K+ flux; guard cells; noninvasive micro-test technique
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2020 年 21 卷 9 期
页码：
收录情况： SCI
摘要： Water deficit caused by osmotic stress and drought limits crop yield and tree growth worldwide. Screening and identifying candidate genes from stress-resistant species are a genetic engineering strategy to increase drought resistance. In this study, an increased concentration of mannitol resulted in elevated expression of thioredoxin f (KcTrxf) in the nonsecretor mangrove species Kandelia candel. By means of amino acid sequence and phylogenetic analysis, the mangrove Trx was classified as an f-type thioredoxin. Subcellular localization showed that KcTrxf localizes to chloroplasts. Enzymatic activity characterization revealed that KcTrxf recombinant protein possesses the disulfide reductase function. KcTrxf overexpression contributes to osmotic and drought tolerance in tobacco in terms of fresh weight, root length, malondialdehyde (MDA) content, and hydrogen peroxide (H2O2) production. KcTrxf was shown to reduce the stomatal aperture by enhancing K+ efflux in guard cells, which increased the water-retaining capacity in leaves under drought conditions. Notably, the abscisic acid (ABA) sensitivity was increased in KcTrxf-transgenic tobacco, which benefits plants exposed to drought by reducing water loss by promoting stomatal closure. KcTrxf-transgenic plants limited drought-induced H2O2 in leaves, which could reduce lipid peroxidation and retain the membrane integrity. Additionally, glutathione (GSH) contributing to reactive oxygen species (ROS) scavenging and transgenic plants are more efficient at regenerating GSH from oxidized glutathione (GSSG) under conditions of drought stress. Notably, KcTrxf-transgenic plants had increased glucose and fructose contents under drought stress conditions, presumably resulting from KcTrxf-promoted starch degradation under water stress. We conclude that KcTrxf contributes to drought tolerance by increasing the water status, by enhancing osmotic adjustment, and by maintaining ROS homeostasis in transgene plants.
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