A single amino acid in coat protein of Pepper mild mottle virus determines its subcellular localization and the chlorosis symptom on leaves of pepper
文献类型： 外文期刊
作者： Han, Kelei 1 ; Zheng, Hongying 2 ; Ji, Mengfei 2 ; Cui, Weijun 2 ; Hu, Shuzhen 1 ; Peng, Jiejun 2 ; Zhao, Jinping 2 ; Lu, 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China
2.Ningbo Univ, State Key Lab Managing Biot & Chem Threats Qual &, Inst Plant Virol, Ningbo 315211, Peoples R China
3.Hunan Inst Plant Protect, Changsha 410125, Peoples R China
4.Zhejiang Acad Agr Sci, Key Lab Biotechnol Plant Protect MOA & Zhejiang P, Hangzhou 310021, Peoples R China
关键词： Pepper mild mottle virus; coat protein; subcellular localization; chlorosis
期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）
ISSN： 0022-1317
年卷期： 2020 年 101 卷 5 期
页码：
收录情况： SCI
摘要： Pepper mild mottle virus (PMMoV) causes serious economic losses in pepper production in China. In a survey for viral diseases on pepper, two PMMoV isolates (named PMMoV-ZJ1 and PMMoV-ZJ2) were identified with different symptoms in Zhejiang province. Sequence alignment analysis suggested there were only four amino acid differences between the isolates: Val262Gly, Ile629Met and Ala1164Thr in the replicase, and Asp20Asn in the coat protein. Infectious cDNA clones of both isolates were constructed and shown to cause distinctive symptoms. Chlorosis symptoms appeared only on PMMoV-ZJ2-infected plants and the Asp20Asn substitution in the CP was shown to be responsible. Confocal assays revealed that the subcellular localization pattern of the two CPs was different, CP20Asp was mainly located at the cell periphery, whereas most CP20Asn located in the chloroplast. Thus, a single amino acid in the CP determined the chlorosis symptom, accompanied by an altered subcellular localization.
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