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Structural features and antiviral function of the MDA5 gene in ducks (Anas platyrhynchos)

文献类型： 外文期刊

作者： Gu, Tiantian 1 ; Li, Guoqin 1 ; Tian, Yong 1 ; Chen, Li 1 ; Wu, Xin-Sheng 2 ; Zeng, Tao 1 ; Xu, Qi 2 ; Spyrydonov, Vladys 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Hangzhou 310021, Peoples R China

2.Yangzhou Univ, Jiangsu Key Lab Anim Genet Breeding & Mol Design, Yangzhou 225009, Jiangsu, Peoples R China

3.Minist Agr China, Key Lab Informat Traceabil Agr Prod, Hangzhou 310021, Peoples R China

4.Natl Univ Life & Environm Sci Ukraine, UA-03041 Kiev, Ukraine

关键词： duck; MDA5; RNAi; gene expression; poly(I:C)

期刊名称：CANADIAN JOURNAL OF ANIMAL SCIENCE （ 影响因子：1.015； 五年影响因子：1.094 ）

ISSN： 0008-3984

年卷期： 2020 年 100 卷 2 期

页码：

收录情况： SCI

摘要： Melanoma differentiation-associated gene 5 (MDA5) is an important cytoplasmic RNA sensor that detects viral double-stranded RNA in innate immunity. The objective of this study was to characterize the structure and function of the MDA5 gene in the duck. In this study, full-length duck MDA5 (duMDA5) complementary DNA (cDNA) was obtained using the reverse transcription-polymerase chain reaction and rapid amplification of the cDNA ends. The cDNA consisted of a 123 nucleotide 5' untranslated region (UTR), a 735 nucleotide 3' UTR, and a 3012 nucleotide open-reading frame, encoding 1003 amino acids. Multiple sequence alignments showed that duMDA5 had 91.18% and 83.11% amino acid sequence similarity with geese and chicken MDA5, respectively, as well as 59.76%-61.26% sequence identity with mammalian homologs. Phylogenetic analysis demonstrated that MDA5 has been highly conserved throughout vertebrate evolution. Quantitative real-time polymerase chain reaction analysis indicated that the duMDA5 mRNA is scarcely detected in healthy tissues and the highest relative transcript level of duMDA5 was induced during poly(I:C) stimulation. Furthermore, knockdown duMDA5 significantly inhibited the transcription of poly(I:C)-induced beta interferons, nuclear factor kappa-B, interferon regulatory factor 7, translocated intimin receptor domain-containing adaptor protein inducing beta interferons, interferon-induced GTP-binding protein, signal transducer and activator of transcription 1 and 2 mRNA. Taken together, these results suggest that duMDA5 is an important receptor for inducing antiviral activity in the duck's innate immune response.

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