您好,欢迎访问福建省农业科学院 机构知识库!

Exploring the Distribution of Blast Resistance Alleles at the Pi2/9 Locus in Major Rice-Producing Areas of China by a Novel Indel Marker

文献类型: 外文期刊

作者: Tian, Dagang 1 ; Lin, Yan 1 ; Chen, Ziqiang 1 ; Chen, Zaijie 1 ; Yang, Fang 2 ; Wang, Feng 1 ; Wang, Zonghua 2 ; Wang, Mo 2 ;

作者机构: 1.Fujian Acad Agr Sci, Biotechnol Res Inst, Fujian Key Lab Genet Engn Agr, Fuzhou 350003, Fujian, Peoples R China

2.Fujian Agr & Forestry Univ, State Key Lab Ecol Pest Control Fujian & Taiwan C, Coll Life Sci, Fuzhou 350002, Fujian, Peoples R China

3.Fujian Agr & Forestry Univ, Fujian Univ, Key Lab Plant Microbe Interact, Fuzhou 350002, Fujian, Peoples R China

4.Minjiang Univ, Inst Oceanog, Fuzhou 350108, Fujian, Peoples R China

关键词: blast disease; InDel marker; Pigm; Pi9; Pi2; Piz-t; rice

期刊名称:PLANT DISEASE ( 影响因子:4.438; 五年影响因子:4.7 )

ISSN: 0191-2917

年卷期: 2020 年 104 卷 7 期

页码:

收录情况: SCI

摘要: Rice blast disease caused by the fungus Magnaporthe oryzae damages cereal crops and poses a high risk to rice production around the world. Currently, planting cultivars with resistance (R) genes is still the most environment-friendly approach to control this disease. Effective identification of R genes existing in diverse rice cultivars is important for understanding the distribution of R genes and predicting their contribution to resistance against blast isolates in regional breeding. Here, we developed a new insertion/deletion (InDel) marker, Pigm/2/9InDel, that can differentiate the cloned R genes (Pigm, Pi9, and Pi2/Piz-t) at the Pi2/9 locus. Pigm/2/9InDel combined with the marker Pi2-LRR for Pi2 was applied to determine the distribution of these four R genes among 905 rice varieties, most of which were collected from the major rice-producing regions in China. In brief, nine Pigm-containing varieties from Fujian and Guangdong provinces were identified. All of the 62 Pi2-containing varieties were collected from Guangdong, and 60 varieties containing Piz-t were from seven provinces. However, Pi9 was not found in any of the Chinese varieties. The newly identified varieties carrying the Pi2/9 alleles were further subjected to inoculation tests with regional blast isolates and field trials. Our results indicate that Pigm and Pi2 alleles have been introgressed for blast resistance breeding mainly in the Fujian and Guangdong region, and Pi9 is a valuable blast resistance resource to be introduced into China.

  • 相关文献
作者其他论文 更多>>