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Chemometrics-enhanced HPLC-DAD as a rapid and interference-free strategy for simultaneous quantitative analysis of flavonoids in Chinese propolis

文献类型： 外文期刊

作者： Zhang, Xiao-Hua 1 ; Ma, Yi-Xin 1 ; Yi, Chong 1 ; Qing, Xiang-Dong 2 ; Liu, Zhi 3 ; Zheng, Jing-Jing 1 ; Lin, Fang 1 ; Lv, 1 ;

作者机构： 1.Xuchang Univ, Henan Key Lab Biomarker Based Rapid Detect Techno, Food & Pharm Coll, Xuchang 461000, Peoples R China

2.Hunan City Univ, Hunan Prov Key Lab Dark Tea & Jin Hua, Coll Mat & Chem Engn, Yiyang 413049, Peoples R China

3.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Inst Qual & Stand Agr Prod, Hangzhou 310021, Peoples R China

关键词： Flavonoids; Propolis; Alternating trilinear decomposition; HPLC-DAD; Second-order multivariate methods

期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY （ 影响因子：2.998； 五年影响因子：3.005 ）

ISSN： 1438-2377

年卷期： 2020 年 246 卷 10 期

页码：

收录情况： SCI

摘要： In this paper, the application of chemometrics coupled with high-performance liquid chromatography-diode array detection (HPLC-DAD) for the rapid and sensitive quantitative analysis of seven flavonoids in the raw and purified propolis samples was investigated. The objective of this paper was to receive a successful resolution of the complex propolis matrix with minimum experimental time (3.5 min) when appearing unknown interferences, highly overlapping peaks, time and baseline drifts among chromatograms. First, the validation samples contained seven flavonoids at five levels, helped to understand and interpret the features of the second-order calibration method based on the alternating trilinear decomposition (ATLD) algorithm. Second, the experimental data were studied for the simultaneous determination of flavonoids in the raw and purified propolis samples using the function of "mathematical separation" of ATLD algorithm. Except for myricetin (MY), the average recoveries of other analytes were 95.79-105.82% for the raw propolis samples, 94.41-108.06% for the purified propolis samples. In addition, the limit of detection (LOD, 0.01-0.20 mu g mL(-1)), limit of quantification (LOQ, 0.03-0.60 mu g mL(-1)), sensitivity (SEN, 15.97-184.31 mL mu g(-1)), selectivity (SEL, 0.16-0.76) and root-mean-squared error of prediction (RMSEP, 0.29-1.64 mu g mL(-1)) of the proposed method, demonstrated that all the target analytes have good sensitivity, low detection limit. According to the experimental results, the total content of flavonoids in purified propolis is about twice that in raw propolis, which is consistent with the actual situation. This analytical strategy will be extremely promising for the identification and quantification of analytes studied in the complex samples.

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