文献类型： 外文期刊
作者： Gu, Tiantian 1 ; Li, Guoqin 1 ; Wu, Xinsheng 2 ; Zeng, Tao 1 ; Xu, Qi 2 ; Li, Liumeng 3 ; Vladyslav, Spyrydonov 4 ; Chen, 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Hangzhou 310000, Zhejiang, Peoples R China
2.Yangzhou Univ, Jiangsu Key Lab Anim Genet Breeding & Mol Design, Yangzhou 225009, Jiangsu, Peoples R China
3.Zhuji Poultry Dev Co Ltd, Zhuji, Zhejiang, Peoples R China
4.Natl Univ Life & Environm Sci Sci Ukraine, Kiev, Ukraine
关键词： Duck; toll-like receptor 7; gene; expression
期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：2.282； 五年影响因子：1.702 ）
ISSN： 1049-5398
年卷期：
页码：
收录情况： SCI
摘要： Toll-like receptors (TLRs) play an important role in detecting pathogen-associated molecular patterns (PAMPs). Among the TLRs, TLR7 is involved in the recognition of antiviral compounds and single-stranded RNA. This study was designed to explore the structure and function of TLR7 in duck (Anas platyrhynchos), a natural host for avian influenza virus. Firstly, the full-length cDNA of Shaoxing egg-laying duck TLR7 (duTLR7) was obtained using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). It consisted of 38 base pairs (bp) 5 '-untranslated region (UTR), 187 bp 3 '-UTR, and 3270 bp open reading frame that encodes a single protein of 1089 amino acid residues. DuTLR7 shares high identity with TLR7 genes from other vertebrates. In healthy ducks, duTLR7 transcripts were broadly expressed in different tissues, with higher expression levels in the liver, kidney, and thymus. The highest relative transcript level of duTLR7 could be induced with R848 stimulation. In addition, overexpression of duTLR7 by stimulating with poly(I:C) significantly promoted IFN-beta, NF-kappa B, IRF7, TRIF, Mx, STAT1 and STAT2 expressions. Taken together, these results suggest that TLR7 may play an important role in the innate immune response of ducks.
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