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Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values

文献类型： 外文期刊

作者： Huo, Jinxi 1 ; Zhong, Shi 1 ; Du, Xin 1 ; Cao, Yinglong 2 ; Wang, Wenqiong 2 ; Sun, Yuqing 1 ; Tian, Yu 4 ; Zhu, Jianxun; 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Peoples R China

2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Pharmacol & Toxicol Res Ctr, Beijing 100193, Peoples R China

关键词： Anti-carcinogenesis activity; Genome sequencing; HR-ESI-MS; Inoscavin A; Phellinus gilvus; Phenylpropanoids biosynthesis

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：10.479； 五年影响因子：9.479 ）

ISSN： 2090-1232

年卷期： 2020 年 24 卷

页码：

收录情况： SCI

摘要： Phellinus gilvus (Schwein.) Pat, a species of 'Sanghuang', has been well-documented for various medicinal uses, but the genome information and active constituents are largely unknown. Here, we sequenced the whole-genome of P. gilvus, identified phenylpropanoids as its key anti-cancer components, and deduced their biosynthesis pathways. A 41.11-Mb genome sequence was assembled and the heatmap created with high-throughput chromosome conformation capture techniques data suggested all bins could be clearly divided into 11 pseudochromosomes. Cellular experiments showed that P. gilvus fruiting body was more effective to inhibit hepatocellular carcinoma cells than mycelia. High resolution electrospray ionization mass spectroscopy (HR-ESI-MS) analysis revealed P. gilvus fruiting body was rich in phenylpropanoids, and several unique phenylpropanoids in Phellinus spp. exhibited potent anti-carcinogenesis activity. Based on genomic, HR-ESI-MS information and differentially expressed genes in transcriptome analysis, we deduced the biosynthesis pathway of four major phenylpropanoids in P. gilvus. Transcriptome analysis revealed the deduced genes expressions were synergistically changed with the production of phenylpropanoids. The optimal candidate genes of phenylpropanoids' synthesis pathway were screened by molecular docking analysis. Overall, our results provided a high-quality genomic data of P. gilvus and inferred biosynthesis pathways of four phenylpropanoids with potent anti-carcinogenesis activities. These will be a valuable resource for further genetic improvement and effective use of the P. gilvus. (C) 2020 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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