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Characterization and target gene analysis of microRNAs in the antennae of the parasitoid waspMicroplitis mediator

文献类型： 外文期刊

作者： Shan, Shuang 1 ; Wang, Shan-Ning 1 ; Song, Xuan 1 ; Khashaveh, Adel 1 ; Lu, Zi-Yun 5 ; Dhiloo, Khalid Hussain 6 ; Li, 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.China Agr Univ, Coll Plant Protect, Beijing, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing, Peoples R China

4.Agr Univ Hebei, Coll Plant Protect, Baoding, Hebei, Peoples R China

5.Hebei Acad Agr & Forestry Sci, Plant Protect Inst, Minist Agr,IPM Ctr Hebei Prov, Key Lab Integrated Pest Management Crops Northern, Baoding, Hebei, Peoples R China

6.Sindh Agr Univ, Fac Crop Protect, Dept Entomol, Tandojam, Pakistan

关键词： expression profile; miRNA; Microplitis mediator; olfactory-associated gene; target gene

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期：

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs), a class of non-coding single-strand RNA molecules encoded by endogenous genes, are about 21-24 nucleotides long and are involved in the post-transcriptional regulation of gene expression in plants and animals. Generally, the types and quantities of miRNAs in the different tissues of an organism are diverse, and these divergences may be related to their specific functions. Here we have identified 296 known miRNAs and 46 novel miRNAs in the antennae of the parasitoid waspMicroplitis mediatorby high-throughput sequencing. Thirty-three miRNAs were predicted to target olfactory-associated genes, including odorant binding proteins (OBPs), chemosensory proteins, odorant receptors (ORs), ionotropic receptors (IRs) and gustatory receptors. Among these, 17 miRNAs were significantly highly expressed in the antennae, four miRNAs were highly expressed both in the antennae and head or wings, while the remaining 12 miRNAs were mainly expressed in the head, thorax, abdomen, legs and wings. Notably, miR-9a-5p and miR-2525-3p were highly expressed in male antennae, whereas miR-1000-5p and novel-miR-13 were enriched in female antennae. The 17 miRNAs highly expressed in antennae are likely to be associated with olfaction, and were predicted to target one OBP (targeted by miR-3751-3p), one IR (targeted by miR-7-5p) and 14 ORs (targeted by 15 miRNAs including miR-6-3p, miR-9a-5p, miR-9b-5p, miR-29-5p, miR-71-5p, miR-275-3p, miR-1000-5p, miR-1000-3p, miR-2525-3p, miR-6012-3p, miR-9719-3p, novel-miR-10, novel-miR-13, novel-miR-14 and novel-miR-28). These candidate olfactory-associated miRNAs are all likely to be involved in chemoreception through the regulation of chemosensory gene expression in the antennae ofM. mediator.

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