文献类型: 外文期刊
作者: Chen, Liyu 1 ; Nan, Haiyang 1 ; Kong, Lingping 1 ; Yue, Lin 1 ; Yang, Hui 1 ; Zhao, Qingsong 2 ; Fang, Chao 1 ; Li, Haiya 1 ;
作者机构: 1.Guangzhou Univ, Innovat Ctr Mol Genet & Evolut, Sch Life Sci, Guangzhou 510006, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Key Lab Crop Genet & Breeding Hebei, Shijiazhuang 050000, Hebei, Peoples R China
3.Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210000, Peoples R China
4.Chinese Acad Sci, Innovat Acad Seed Design, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Harbin 150000, Peoples R China
期刊名称:JOURNAL OF INTEGRATIVE PLANT BIOLOGY ( 影响因子:7.061; 五年影响因子:6.002 )
ISSN: 1672-9072
年卷期:
页码:
收录情况: SCI
摘要: Flowering time and plant height are key agronomic traits that directly affect soybean (Glycine max) yield.APETALA1(AP1) functions as a class A gene in the ABCE model for floral organ development, helping to specify carpel, stamen, petal, and sepal identities. There are fourAP1homologs in soybean, all of which are mainly expressed in the shoot apex. Here, we used clustered regularly interspaced short palindromic repeats (CRISPR) - CRISPR-associated protein 9 technology to generate a homozygous quadruple mutant,gmap1, with loss-of-function mutations in all fourGmAP1genes. Under short-day (SD) conditions, thegmap1quadruple mutant exhibited delayed flowering, changes in flower morphology, and increased node number and internode length, resulting in plants that were taller than the wild type. Conversely, overexpression ofGmAP1aresulted in early flowering and reduced plant height compared to the wild type under SD conditions. Thegmap1mutant and the overexpression lines also exhibited altered expression of several genes related to flowering and gibberellic acid metabolism, thereby providing insight into the role ofGmAP1in the regulatory networks controlling flowering time and plant height in soybean. Increased node number is the trait with the most promise for enhancing soybean pod number and grain yield. Therefore, the mutant alleles of the fourAP1homologs described here will be invaluable for molecular breeding of improved soybean yield.
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