文献类型： 外文期刊

作者： Duan, Y. ¹ ; Chen, J. ² ; Pang, Z. ¹ ; Ye, X. ² ; Zhang, C. ² ; Hu, H. ¹ ; Xie, J. ¹ ;

作者机构： 1.Chinese Acad Trop Agr Sci, Key Lab Trop Fruit Biol, Minist Agr, South Subtrop Crop Res Inst, Zhanjiang 524091, Peoples R China

2.Hainan Univ, Coll Food Sci & Technol, Haikou, Hainan, Peoples R China

关键词： antimicrobials; cell injury; mechanism of action; plant diseases; Streptomycetes

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：3.772； 五年影响因子：3.963 ）

ISSN： 1364-5072

年卷期：

页码：

收录情况： SCI

摘要： Aim Research on prevention and cure of banana wilt is important to ensure the healthy development of the banana industry. In this study, antifungal mechanism ofStreptomycesma.FS-4on fusarium wilt of banana was investigated. Methods and Results The physiological strain of banana fusarium pathogenFusarium oxysporumf. sp.cubense Race4 (FOC.4) was used as the target fungus, and the antifungal mechanism of the crude extract ofStreptomycesma.FS-4was investigated. Eighteen different compounds identified by gas chromatography-mass spectrometry were composed of aldehydes, methyl, hydrocarbons, amides, esters and acids.FS-4significantly inhibited the spore germination of the target fungi, with an EC(50)of 22 center dot 78 mu g ml(-1). After treatment with 100 mu g ml(-1)FS-4crude extract, the N-acetylglucosamine content in the mycelium increased 1 center dot 95-fold. However, the extract had no significant effect on beta-1,3-glucanase. At theFS-4crude extract dose of 100 mu g ml(-1), the total sugar and protein contents decreased by 28 center dot 6 and 29 center dot 1% respectively, and the fat content was 41 center dot 3%.FS-4significantly inhibited the activity of the mitochondrial complex III of Foc4, which was reduced by 52 center dot 45%. MoreoverFS-4reduced the activity of succinate dehydrogenase, a key enzyme in the Krebs cycle, by 60 center dot 2%. However,FS-4had no significant effect on malate dehydrogenase. The membrane potential on the mitochondrial inner membrane was significantly reduced at the test concentration of 100 mu g ml(-1). ROS gradually accumulated in the Foc4 hypha, and the burst was 3 center dot 97 times higher than the control. Conclusions This study demonstrated that the antifungal mechanism ofStreptomycesma.FS-4against Foc4 includes the destruction of the plasma membrane and mitochondrial dysfunction and finally induction of cell apoptosis. Significance and Impact of the Study These results may indicate the prevention and control of banana wilt, which is of great significance to the healthy development of banana industry system.