Development of a Low-Cost and High-Efficiency Culture Medium for Bacteriocin Lac-B23 Production byLactobacillus plantarumJ23
文献类型： 外文期刊
作者： Zhang, Jianming 1 ; Bu, Yushan 2 ; Zhang, Chengcheng 1 ; Yi, Huaxi 2 ; Liu, Daqun 1 ; Jiao, Jingkai 3 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310016, Peoples R China
2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266100, Peoples R China
3.Bright Dairy & Food Co Ltd, State Key Lab Dairy Biotechnol, Dairy Res Inst, Shanghai 200436, Peoples R China
关键词： bacteriocin Lac-B23; low-cost; high-efficiency; culture medium
期刊名称：BIOLOGY-BASEL （ 影响因子：5.079； ）
ISSN：
年卷期： 2020 年 9 卷 7 期
页码：
收录情况： SCI
摘要： At present, De Man, Rogosa and Sharpe (MRS) broth is the medium of choice for promoting bacteriocin production. However, this medium is expensive and not applicable for large-scale production. Therefore, a low-cost and high-efficiency culture medium for bacteriocin Lac-B23 production byLactobacillus plantarumJ23 was developed. First, the effects of the composition of MRS broth on bacteriocin Lac-B23 production and bacterial growth were researched by a one variable at a time approach. Then, a Plackett-Burman design was used to screen significant components for production. Finally, the steepest ascent and central composite designs were used to obtain an optimum medium. The final composition of the modified MRS was much simpler than MRS broth, and the modified MRS contained only glucose, yeast extract, dipotassium phosphate, manganese sulfate monohydrate, Tween 80 and sodium acetate anhydrous. The highest bacteriocin Lac-B23 production reached 2560 activity units (AU)/mL in the modified MRS, which is nine times higher than that in MRS broth (280 AU/mL). Meanwhile, the cost per liter of the modified MRS (8.56 Ren Min Bi (RMB)/L) is 34.70% the cost of MRS broth (13.11 RMB/L), and the cost per arbitrary units of bacteriocin Lac-B23 in the modified MRS is approximately fourteen times more convenient (3.34 RMB/10(6)AU) than in the MRS broth (46.82 RMB/10(6)AU).
- 相关文献
作者其他论文 更多>>
-
Unraveling the metabolite profiles and microbial roles during dry-pickled radish fermentation
作者：Niu, Haiyue;Zhang, Jianming;Zhang, Chengcheng;Xin, Xiaoting;Liu, Daqun;Niu, Haiyue
关键词：Bacterial communities; Volatile metabolites; Non-volatile metabolites; Key enzymes; Fermented radish
-
Enhancing the formation of functional glucosinolate degradation products in fermented broccoli stalk by-product with lactic acid bacteria
作者：Wu, Qinghang;Liu, Daqun;Zhang, Jianming;Li, Ting;Niu, Haiyue;Xin, Xiaoting;Zhang, Chengcheng;Wu, Qinghang;Zhao, Shengming;He, Chengyun
关键词：Broccoli stalk by-product; Glucosinolates; Isothiocyanates; Degradation products; Lactic acid bacteria
-
Different Efficacy of Five Soluble Dietary Fibers on Alleviating Loperamide-Induced Constipation in Mice: Influences of Different Structural Features
作者：Zhang, Zhiguo;Liu, Buyu;Zhang, Chengcheng;Hu, Weiwei;Wu, Weicheng;Liu, Buyu;Liu, Xingquan;Liu, Wei
关键词：soluble dietary fiber; structural difference; constipation; defecation function; short-chain fatty acid; gut microbiota
-
Biotransformation of inorganic selenium into selenium nanoparticles and organic selenium by Lactiplantibacillus plantarum CXG4
作者：Li, Yilong;Wang, Ting;Yi, Huaxi;Li, Yilong;Wang, Ting;Liu, Daqun;Zhang, Chengcheng;Wu, Weicheng;Zhang, Jianming
关键词：Biotransformation; Selenium; Lactiplantibacillus plantarum; Selenite reduction production; Transcriptome analysis
-
Coriander-Derived Exosome-Like Nanovesicles Laden Hydrogel with Antioxidant Property Accelerates Wound Healing
作者：Wang, Ting;Li, Yilong;Hao, Linlin;Liu, Yinxue;Yi, Huaxi;Liu, Daqun;Zhang, Chengcheng;Zhang, Jianming
关键词：coriander; exosome-like nanovesicles; hydrogel; oxidative stress; wound healing
-
Mechanistic insights into the impact of thermally induced endogenous β-glucan structural modifications on the digestibility of highland barley flour
作者：Hu, Weiwei;Gu, Junchao;Zhang, Zhiguo;Liu, Daqun;Wu, Weicheng;Gu, Junchao;Yang, Kai
关键词：Thermal processing; Highland barley; Microstructure; In vitro digestibility; beta-glucan modification
-
Whole-genome analysis, evaluation and regulation of in vitro and in vivo GABA production from Levilactobacillus brevis YSJ3
作者：Liu, Daiyao;Zhang, Chengcheng;Niu, Haiyue;Xin, Xiaoting;Liu, Daqun;Zhang, Jianming;Liu, Hui;Liu, Daiyao;Yi, Huaxi
关键词：GABA-producing bacteria; Levilactobacillus brevis YSJ3; Fermentation kinetics; Metabolism analysis; Improve sleep
