Characterization of flupyradifurone resistance in the whiteflyBemisia tabaciMediterranean (Q biotype)
文献类型： 外文期刊
作者： Wang, Ran 1 ; Wang, Jinda 2 ; Zhang, Jiasong 2 ; Che, Wunan 3 ; Feng, Honglin 4 ; Luo, Chen 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
2.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, Fuzhou, Peoples R China
3.Shenyang Agr Univ, Dept Pesticide Sci, Shenyang, Peoples R China
4.Boyce Thompson Inst Plant Res, Ithaca, NY USA
关键词： Bemisia tabaci; flupyradifurone; metabolic resistance; cytochrome P450s monooxygenase; glutathioneS-transferase; RNA interference
期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）
ISSN： 1526-498X
年卷期： 2020 年 76 卷 12 期
页码：
收录情况： SCI
摘要： BACKGROUND Bemisia tabaciis one of most notorious pests on various crops worldwide and many populations show high resistance to different types of insecticides. Flupyradifurone is a novel insecticide against sucking pests.B. tabaciresistance to flupyradifurone has been detected in the field, however the mechanism of flupyradifurone resistance has rarely been studied. RESULTS The flupyradifurone-resistant strain (FLU-SEL) was selected from the susceptible strain ofB. tabaci(MED-S) using flupyradifurone for 24 generations. The FLU-SEL strain exhibited 105.56-fold resistance to flupyradifurone, and moderate cross-resistance to imidacloprid, but no cross-resistance to other tested neonicotinoids. Synergism tests and metabolic enzyme assays suggested that FLU-SEL resistance can be attributed to enhanced detoxification mediated by glutathioneS-transferase (GST) and P450 monooxygenase (P450). Compared with MED-S strain, CYP6CX4 and GSTs2 were significantly overexpressed in FLU-SEL, and silencing CYP6CX4 or GSTs2 increased the mortality of whiteflies to flupyradifurone challenge in FLU-SEL. In addition, silencing CYP6CX4 also increased the mortality of whiteflies exposed to imidacloprid. CONCLUSION Overexpression of CYP6CX4 and GSTs2 was associated with flupyradifurone resistance, as confirmed by RNA interference. Our findings suggested that metabolic resistance to flupyradifurone might be mediated by P450s and GSTs.
- 相关文献
作者其他论文 更多>>
-
Sublethal and transgenerational effects of tetraniliprole on the tomato pinworm Phthorimaea (=Tuta) absoluta (Lepidoptera: Gelechiidae)
作者：Yao, Jiaqi;Wang, Yuguang;Yao, Jiaqi;Wang, Yuguang;Yao, Jiaqi;Qu, Cheng;Li, Yunyi;Luo, Chen;Wang, Ran;Guedes, Raul Narciso C.
关键词：Phthorimaea absoluta; Tetraniloprole; Transgenerational sublethal effects; Vitellogenin; Vitellogenin receptor; Antioxidant enzymes
-
Salivary Protein Sfapyrase of Spodoptera frugiperda Stimulates Plant Defence Response
作者：Yao, Yang;Lin, Huan-tai;Chen, Yao-hui;Chen, Li-lan;Zhang, Hui-li;Fu, Hua-ying;Gao, San-ji;Wang, Jin-da;Wang, Ran;Feng, Hong-lin
关键词：apyrase; benzoxazinoids; jasmonic acid; plant defence; salivary elicitor; Spodoptera frugiperda
-
Involvement of the cytochrome P450 genes CYP6DW3 and CYP4C64 in afidopyropen resistance in Bemisia tabaci Mediterranean (Q biotype)
作者：Ma, Lijun;Luo, Chen;Tang, Juan;Zhang, Qinghe;Gao, Bingli;Qu, Cheng;Wang, Ran
关键词：Bemisia tabaci; afidopyropen; insecticide detoxification; cytochrome P450 monooxygenase
-
Baseline susceptibility, risk assessment, biochemical mechanism and fitness cost of broflanilide resistance in Tuta absoluta from China
作者：Qu, Cheng;Lin, Ao-li;Wang, Ze-hua;Feng, Yao-fang;Luo, Chen;Wang, Ran;Qu, Cheng;Wang, Ze-hua;Luo, Chen;Wang, Ran;Lin, Ao-li;Fang, Yong;Wang, Ran;Guedes, Raul Narciso C.
关键词：tomato pinworm; toxicity; field-evolved resistance; resistance risk; detoxifying enzymes
-
UHPLC-MS/MS analysis of the influence of paclobutrazol application on Ophiopogon japonicus
作者：Zhao, Haoran;Zhang, Zezhou;Xiao, Ouli;Chen, Jieyin;Dai, Xiaofeng;Kong, Zhiqiang;Chen, Jieyin;Li, Minmin;Zhao, Haoran;Wang, Ran
关键词：Growth regulators; Ophiopogon japonicus; Saponins; Flavonoids
-
Life-History Parameters of Phyllotreta striolata (F.) (Coleoptera: Chrysomelidae) Acquired by a Laboratory-Rearing Method
作者：Wei, Yiyun;Chen, Bin;Tian, Panpan;Wei, Yiyun;Zhou, Jincheng;Luo, Chen
关键词：
Phyllotreta striolata ; laboratory-rearing method; life-history parameters -
Residue behaviors and dietary risk assessment of dinotefuran and its metabolites in Lycium barbarum from farm to fork
作者：Xiao, Ouli;Chen, Jieyin;Dai, Xiaofeng;Kong, Zhiqiang;Li, Minmin;Chen, Jieyin;Dai, Xiaofeng;Kong, Zhiqiang;Wang, Ran;Dai, Xiaofeng;Kong, Zhiqiang
关键词：Wolfberry; Dinotefuran; Dissipation behavior; Dietary risk assessment; Bioaccessibility
