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Threepoint-mutationsin cytochromebconfer resistance to trifloxystrobin inMagnaporthe oryzae

文献类型： 外文期刊

作者： Miao, Jianqiang 1 ; Zhao, Guosen 1 ; Wang, Bin 2 ; Du, Yixin 4 ; Li, Zhiwen 5 ; Gao, Xuheng 1 ; Zhang, Can 2 ; Liu, Xili 1 ;

作者机构： 1.Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 772700, Shaanxi, Peoples R China

2.China Agr Univ, Dept Plant Pathol, Beijing, Peoples R China

3.Shenyang Sinochem Agrochem R&D Co Ltd, State Key Lab Discovery & Dev Novel Pesticide, Shenyang, Peoples R China

4.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou, Peoples R China

5.Inst Control Agrochem Shaanxi Prov, Xian, Peoples R China

关键词： trifloxystrobin; QoI fungicide; Magnaporthe oryzae; resistance; G143S; G137R; M296V

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2020 年 76 卷 12 期

页码：

收录情况： SCI

摘要： BACKGROUND Rice blast, caused byMagnaporthe oryzae, is the most devastating disease in rice. Recently, trifloxystrobin was registered for the control ofM. oryzaein China. The resistance profile and mechanism ofM. oryzaeto trifloxystrobin were investigated in the present study, providing important data for the recommended use of trifloxystrobin. RESULTS The baseline sensitivity was established at a half maximal effective concentration (EC50) of 0.024 mu g mL(-1). Nine stable trifloxystrobin-resistant mutants were generated with EC(50)values ranging from 12.75 to 171.49 mu g mL(-1). The mutants exhibited strong adaptive traits in sporulation, conidial germination, and pathogenicity. Positive cross-resistance was only observed between trifloxystrobin and azoxystrobin, but not between trifloxystrobin and carbendazim, isoprothiolane, prochloraz, or chlorothalonil. The point mutation G143S in cytochrome b (cytb) protein was found in eight high-resistance mutants with resistant factor ranging from 2295.16 to 13 200.00; and the double mutation G137R/M296V only occurred in Mg117-1 with resistance factor approximate to 900. The G143S mutation weakened hydrogen bond interactions, and G137R/M296V changed the conformation of trifloxystrobin in the cytbbinding pocket. A molecular detection method was established for the rapid detection of G143S mutants inM. oryzae. CONCLUSION The resistance risk ofM. oryzaeto trifloxystrobin could be moderate to high. Two genotypes with three point-mutations G143S, G137R, and M296V conferred resistance to trifloxystrobin inM. oryzae.

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