Genome-wide identification, transcriptome analysis and alternative splicing events of Hsf family genes in maize
文献类型： 外文期刊
作者： Zhang, Huaning 1 ; Li, Guoliang 1 ; Fu, Cai 1 ; Duan, Shuonan 1 ; Hu, Dong 1 ; Guo, Xiulin 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Plant Genet Engn Ctr Hebei Prov, Inst Genet & Physiol, Shijiazhuang 050051, Hebei, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2020 年 10 卷 1 期
页码：
收录情况： SCI
摘要： Heat shock transcription factor (Hsf) plays a transcriptional regulatory role in plants during heat stress and other abiotic stresses. 31 non-redundant ZmHsf genes from maize were identified and clustered in the reference genome sequenced by Single Molecule Real Time (SMRT). The amino acid length, chromosome location, and presence of functional domains and motifs of all ZmHsfs sequences were analyzed and determined. Phylogenetics and collinearity analyses reveal gene duplication events in Hsf family and collinearity blocks shared by maize, rice and sorghum. The results of RNA-Seq analysis of anthesis and post-anthesis periods in maize show different expression patterns of ZmHsf family members. Specially, ZmHsf26 of A2 subclass and ZmHsf23 of A6 subclass were distinctly up-regulated after heat shock (HS) at post-anthesis stage. Nanopore transcriptome sequencing of maize seedlings showed that alternative splicing (AS) events occur in ZmHsf04 and ZmHsf17 which belong to subclass A2 after heat shock. Through sequence alignment, semi-quantitative and quantitative RT-PCR, we found that intron retention events occur in response to heat shock, and newly splice isoforms, ZmHsf04-II and ZmHsf17-II, were transcribed. Both new isoforms contain several premature termination codons in their introns which may lead to early termination of translation. The ZmHsf04 expression was highly increased than that of ZmHsf17, and the up-regulation of ZmHsf04-I transcription level were significantly higher than that of ZmHsf04-II after HS.
- 相关文献
作者其他论文 更多>>
-
Effect of complex prebiotics on Lactobacillus gasseri JM1 fermented soymilk: Physicochemical, flavor characteristics, and metabolites
作者：Zheng, Yaping;Wu, Wenfang;Zhu, Man;Sun, Yilin;Jiang, Yujun;Zhang, Yu;Man, Chaoxin;Hu, Dong
关键词：Fermented soymilk; Complex prebiotics; Lactobacillus gasseri JM1; Flavor; Metabolites
-
Intron retention via alternative splicing affects the thermotolerance regulation of ZmHsf17
作者：Zhang, Huaning;Meng, Xiangzhao;Ma, Zhenyu;Liu, Ran;Liu, Zihui;Duan, Shuonan;Li, Guoliang;Guo, Xiulin;Li, Ran;Zhang, Wenying
关键词：
-
Molecular characterization of a novel heat shock transcription factor gene TaHsfA2-11 and its overexpression improves thermotolerance in wheat
作者：Li, Guoliang;Liu, Zihui;Zhang, Huaning;Zhao, Baihui;Zhang, Yujie;Ma, Zhenyu;Duan, Shuonan;Meng, Xiangzhao;Guo, Xiulin;Zhao, Baihui;Zhang, Yujie
关键词：Wheat; TaHsfA2-11; Heat shock; Thermotolerance; Heat-response genes
-
Upregulation of Wheat Heat Shock Transcription Factor TaHsfC3-4 by ABA Contributes to Drought Tolerance
作者：Ma, Zhenyu;Zhao, Baihui;Zhang, Huaning;Duan, Shuonan;Liu, Zihui;Guo, Xiulin;Meng, Xiangzhao;Li, Guoliang;Zhao, Baihui
关键词：wheat; drought; abscisic acid; heat shock transcription factors; TaHsfC3-4
-
Up-regulation of growth-related gene expression in tobacco by volatile compounds released by Bacillus velezensis WSW007
作者：He, Yuxi;Peng, Jieli;Jia, Nan;Wang, Xu;Ma, Jia;Zhang, Cuimian;Hu, Dong;Wang, Zhanwu;Wang, Hao;Wang, Entao
关键词：
-
Changes in concentrations and transcripts of plant hormones in wheat seedling roots in response to Fusarium crown rot
作者：Gao, Yutian;Tian, Xuejun;Wang, Weidong;Xu, Xiangru;Su, Yuqing;Yang, Jiatian;Li, Jinlong;Xin, Mingming;Peng, Huiru;Sun, Qixin;Xie, Chaojie;Ma, Jun;Duan, Shuonan
关键词：Fusarium crown rot (FCR); Wheat; Hormone; Root; TaOPR3
-
Boosting wheat functional genomics via an indexed EMS mutant library of KN9204
作者：Wang, Dongzhi;Wang, Haojie;Xu, Yongxin;Yang, Yiman;Zhou, Yuxin;Zhou, Yuqing;Lin, Xuelei;He, Fei;Xiao, Jun;Li, Yongpeng;Zhou, Chunjiang;Tang, Wenqiang;Zheng, Shuzhi;Li, Junming;Liu, Xigang;Li, Yongpeng;Li, Junming;Wang, Haojie;Xu, Yongxin;Zhou, Yuxin;Zhou, Yuqing;He, Fei;Xiao, Jun;Chen, Zhongxu;Gui, Lixuan;Jiao, Yuling;He, Yuehui;Guo, Xiulin;Zhang, Yingjun;Cui, Fa;He, Fei;Xiao, Jun
关键词：wheat; exome capture sequencing; EMS mutagenesis; functional genomics
