Reference gene validation inEotetranychus sexmaculatus(Acari: Tetranychidae) feeding on mite-susceptible and mite-resistant rubber tree germplasms
文献类型： 外文期刊
作者： Liang, Xiao 1 ; Chen, Qing 1 ; Wu, Chunling 1 ; Liu, Ying 1 ; Fang, Yongjun 2 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Rubber Res Inst, Haikou, Hainan, Peoples R China
关键词： Eotetranychus sexmaculatus; Reference gene; Rubber tree germplasms; Validation of gene expression
期刊名称：EXPERIMENTAL AND APPLIED ACAROLOGY （ 影响因子：2.132； 五年影响因子：2.196 ）
ISSN： 0168-8162
年卷期： 2020 年 82 卷 2 期
页码：
收录情况： SCI
摘要： Reliable reference genes are quite important in calculating gene transcript levels by using real-time quantitative reverse transcription-PCR (RT-qPCR).Eotetranychus sexmaculatusis known as a dangerous mite causing significant yield reduction of rubber tree latex; however, selection of appropriate reference genes for validation of target gene expression inE. sexmaculatushas not been conducted yet. In the present study, nine candidate reference genes were analyzed for their expression stability in different life stages ofE. sexmaculatusby using common algorithms including comparative Delta Cq method, geNorm, NormFinder and BestKeeper. In addition, a comprehensive analysis software (RefFinder) was used to assign an overall final rank for each candidate gene. The results showed that beta-actinand beta-TUBwere the best two reference genes and were subjected to evaluate expression of two protective enzyme genes (EsCu/ZnSODandEsCAT1) inE. sexmaculatus. We found that the expression ofEsCu/ZnSODandEsCAT1inE. sexmaculatusfeeding on mite-resistant rubber tree germplasm was significantly lower compared with those feeding on mite-susceptible germplasm. These results will facilitate research in revealing molecular mechanisms underlying rubber tree resistance to the spider mite.
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