您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and expression analysis of theHevea brasiliensisphosphate transporter 1 gene family

文献类型： 外文期刊

作者： Sun, Yong 1 ; Gao, Le 1 ; Wang, Dan 2 ; Wu, Bingsun 1 ; Tong, Zheng 2 ; Wu, Min 1 ; Wang, Guihua 1 ; Wu, Wenguan 1 ; Wei, Ji 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab Biol & Genet Resources Rubber Tree, Minist Agr & Rural Affairs, Haikou, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Hainan, Peoples R China

3.Hainan Univ, Coll Trop Crops, Haikou, Hainan, Peoples R China

关键词： Hevea brasiliensis; Phosphate transporter 1; Phosphorus; Phosphate starvation

期刊名称：TREES-STRUCTURE AND FUNCTION （ 影响因子：2.529； 五年影响因子：2.562 ）

ISSN： 0931-1890

年卷期：

页码：

收录情况： SCI

摘要： Key message TwelveHbPHT1swere identified inHeveaand most of them were induced by phosphate starvation. HbPHT1;5 complemented the defective yeast mutant strain EY917 and HbPHT1;7 only partly complemented it. Phosphorus is an important element of the latex of rubber tree, and its content affects the quality and yield of latex. However, with the outflow of latex, a large amount of phosphorus is lost. The phosphate transporter 1 (PHT1) family proteins regulate the phosphorus acquisition and translocation in the plants, but their expression and roles inHevea brasiliensisremain unclear. In this work, we identified twelveHbPHT1s (HbPHT1;1toHbPHT1;12). Evolutionary analyses suggested two duplication events:HbPHT1;5/HbPHT1;6andHbPHT1;7/HbPHT1;8. In the promoter region of someHbPHT1s, there existed P1BS, W-box, MYB, and ethylene responsecis-elements. All theHbPHT1s, except forHbPHT1;4, were expressed in the roots, and most of them were also expressed in the stems and leaves.HbPHT1;7had the highest expression level in the roots, stems, and leaves.HbPHT1;5andHbPHT1;6had a relatively higher expression levels than other genes in latex.HbPHT1;5,HbPHT1;7, andHbPHT1;11were induced by ethylene treatment. MostHbPHT1swere induced under phosphate starvation conditions in the seedling leaves, and the expression ofHbPHT1;9was 20-fold higher than the control.HbPHT1;5complemented the deficiency of the yeast mutant strain EY917 in phosphate transportation under low- and high-phosphate conditions, andHbPHT1;7restored the deficiency when 10 mM phosphate was supplied. These results suggested that the increased expression of PHT1s may improve the phosphate uptake, transport, and utilization inHevea. It also provides the basis to sudy the phosphorus metabolism network and mine the gene resources for the genetic breeding ofHevea.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台