Rapid and Efficient Detection of 16SrI Group Areca Palm Yellow Leaf Phytoplasma in China by Loop-Mediated Isothermal Amplification
文献类型： 外文期刊
作者： Yu, Shao-shuai 1 ; Che, Hai-yan 2 ; Wang, Sheng-jie 3 ; Lin, Cai-li 4 ; Lin, Ming-xing 1 ; Song, Wei-wei 1 ; Tang, Qin 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Coconut Res Inst, Wenchang 571339, Peoples R China
2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China
3.Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China
4.Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China
关键词： Areca palm yellow leaf disease; loop-mediated isothermal amplification; phytoplasma; rapid detection; 16S rRNA gene sequence
期刊名称：PLANT PATHOLOGY JOURNAL （ 影响因子：1.795； 五年影响因子：2.314 ）
ISSN： 1598-2254
年卷期： 2020 年 36 卷 5 期
页码：
收录情况： SCI
摘要： Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loop-mediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrIB subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64 degrees C for 40 min. The lowest detection limit for the two LAMP detection assays was the same at 200 ag/mu l, namely approximately 53 copies/mu l of the target fragments. Phytoplasma was detected in all AYL disease samples from Baoting, Tunchang, and Wanning counties in Hainan province using the two sets of LAMP primers 16SrD-NA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of diseaseresistant Areca palm varieties.
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