文献类型： 外文期刊

作者： Yang, D. ¹ ; Li, S. ¹ ; Lu, L. ¹ ; Fang, J. ¹ ; Wang, W. ¹ ; Cui, H. ¹ ; Tang, D. ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, State Key Lab Ecol Control Fujian Taiwan Crop Pes, Minist Educ Genet Breeding & Multiple Utilizat Cr, Key Lab,Plant Immun Ctr, Fuzhou 350002, Peoples R China

2.Fujian Acad Agr Sci, Rice Res Inst, Fuzhou 350019, Peoples R China

关键词： breeding application; disease-resistance (R) gene; Pigm; mapping; molecular markers; Rice; rice blast

期刊名称：PLANT BIOLOGY （ 影响因子：3.081； 五年影响因子：2.972 ）

ISSN： 1435-8603

年卷期： 2020 年 22 卷 6 期

页码：

收录情况： SCI

摘要： Rice blast, caused byMagnaporthe oryzae, is one of the most destructive diseases of rice worldwide. The identification and utilization of resistance genes are crucial and significant for breeding resistant rice cultivars. We identified a resistance gene from Shuangkang77009, which is highly resistant to the rice blast isolate Guy11 using map-based cloning. We performed bulked segregant analysis combined with specific length amplified fragment sequencing. We also performed association analysis, candidate gene prediction and cDNA sequencing to identify the candidate gene. The resistance gene is located on chromosome 6, and we ultimately mapped the resistance locus to a 92-kb region. The resistance gene in Shuangkang77009 was allelic toPigmR, hereafter referred to asPigm-1. The Pigm-1 protein had one amino acid change: serine (Ser) residue 860 was replaced by tyrosine (Tyr) in Pigm-1 compared with the previously identified Pigm protein, which significantly changed the structure of the Pigm-1 protein based on 3-D structure simulation. In addition, using the developed molecular marker linked to thePigm-1 gene and molecular marker-assisted selection technology, we introduced thePigm-1gene into Minghui86, a widely used and excellent restorer. We generated 11 stable homozygous rice lines with desirable agronomic traits and strong resistance to rice blast. In conclusion,Pigm-1,a natural allelic variant ofPigmR,was responsible for blast resistance in Shuangkang77009 rice. The molecular marker-assisted breeding strategy forPigm-1was highly efficient.