文献类型： 外文期刊

作者： Zhou, W. Q. ¹ ; Zhou, Y. Q. ¹ ; He, C. Y. ² ; Mou, B. Q. ³ ; Zhou, W. ⁴ ;

作者机构： 1.Gansu Acad Agr Sci, Crop Res Inst, Lanzhou 730070, Peoples R China

2.Gansu Univ Chinese Med, Pharm Dept, Lanzhou 730020, Peoples R China

3.ARS, USDA, Salinas, CA 93905 USA

4.Int Rice Res Inst, Plant Breeding Genet & Biol Div, Genet Transformat Lab, Manila 7777, Philippines

关键词： Oryza sativa; Oshox4; rice; drought stress; salinity stress; over-expression

期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）

ISSN： 1021-4437

年卷期： 2020 年 67 卷 6 期

页码：

收录情况： SCI

摘要： Plant hormones play major roles in abiotic stress. This study shows that gibberellin acid-inefficient transgenic rice (Oryza sativaL.) is more tolerant to drought and salinity stress. We also investigated the underlying molecular mechanism by which plants respond to salinity and drought stress. We previously showed how the HD-Zip family I geneOshox4(Homeobox 4) regulates GA deactivation by controlling DELLA subfamily and rice GA 2-oxidase and GA 3-oxidase genes in rice. Here we investigated the role of riceOshox4gene for drought and salinity stress tolerance. Phenotypic analysis indicated thatOshox4transgenic lines are more tolerant to drought and salinity stress relative to non-transgenic IR64 plants. To explain the strong osmotic stress tolerance ofOshox4transgenic lines, we studied the expression of rice GA 2-oxidase, rice YABBY, rice KRP, and rice GRAS gene families. qRT-PCR results showed thatOsGA2ox4,OsGA2ox6,OsGA2ox8,OsGAIandOsSCRLwere highly expressed under severe drought inOshox4transgenic plants.Oshox4enhanced the expression ofOsGA2ox5,OsKRP2andOsKRP5under 50 mM salt treatment andOsSCRLunder 200 mM salt treatment. Our findings have confirmed thatOshox4transgenic rice plants are more tolerant to drought and salinity stress and thatOshox4plays a role in rice osmatic tolerance. This study provides novel insights into methods of maintaining higher yield amidst a limited supply of water by improving physiological conditions.