文献类型： 外文期刊
作者： Chen, Hua 1 ; Zhang, Lijuan 1 ; Ke, Ling 1 ; Chen, Xu 1 ; Lin, Chentao 2 ;
作者机构： 1.Fujian Acad Agr Sci, Biotechnol Inst, High Tech Bldg 1506,Wusi Rd 247, Fuzhou 350003, Fujian, Peoples R China
2.Fujian Normal Univ, Publ Serv Platform Ind Dev Technol Marine Biol Me, State Ocean Adm, Fuzhou, Peoples R China
3.Fujian Normal Univ, Southern Inst Oceanog, Ctr Engn Technol Res Microalgae Germplasm Improve, Fuzhou, Peoples R China
4.Fujian Normal Univ, Fujian Key Lab Special Marine Bioresources Sustai, Fuzhou, Peoples R China
关键词： CpG-ODN 2007; gene expression; Vibrio vulnificus; zebrafish (Danio rerio); ZF4 cells
期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）
ISSN： 1355-557X
年卷期：
页码：
收录情况： SCI
摘要： Vibrio vulnificusis an aquatic pathogen that can cause primary sepsis and soft tissue infection. CpG oligodeoxynucleotides (CpG-ODN) are a type of essential immunomodulators, which can trigger or enhance immune responses in mammals, fish and humans. In this study, we evaluated the effect of CpG-ODN 2007 as a potential immunostimulant for zebrafish (Danio rerio) infected withV. vulnificusstrain FJ03-X2. Fish injected with the CpG-ODN 2007 showed a lower mortality rate compared with the fish that did not receive treatment; the survival rates of the CpG-ODN 2007-treated group and PBS-treated group were 85% and 57.9% respectively. Also, our in vitro results demonstrated that CpG-ODN 2007 could effectively reduce the toxicity ofV. vulnificusstrain FJ03-X2 to zebrafish embryonic fibroblast (ZF4) cells. Furthermore, we assessed innate immune-related genes (TNF, IL-1 beta, IFNg1-2, MyD88, lysozyme, IL-10, IL-22, IL-26 and TLRs) in expression patterns ofV. vulnificusstrain FJ03-X2 infected zebrafish or ZF4 cells with and without CpG-ODN 2007 treatment. We found that CpG-ODN 2007 could reduce the toxicity ofV. vulnificusto ZF4 cells and regulate the immune system to control the overexpression of specific genes. To sum up, our data indicated that CpG-ODN 2007 protects zebrafish againstVibrio vulnificusinfection.
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