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Development and characterization of novel microsatellite markers for a dominant parasitoid Stethynium empoasca (Hymenoptera: Mymaridae) in tea plantations using high-throughput sequencing

文献类型： 外文期刊

作者： Li, Jinyu 1 ; Shi, Longqing 1 ; Chen, Jie 1 ; You, Minsheng 1 ; You, Shijun 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Inst Appl Ecol, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Peoples R China

2.Minist Educ, Joint Int Res Lab Ecol Pest Control, Fuzhou 350002, Peoples R China

3.Minist Agr, Key Lab Integrated Pest Management Fujian Taiwan, Fuzhou 350002, Peoples R China

4.Fujian Agr & Forestry Univ, Fujian Prov Univ, Key Lab Green Pest Control, Fuzhou 350002, Peoples R China

5.Fujian Acad Agr Sci, Inst Rice, Fuzhou 350001, Peoples R China

关键词： Parasitoid; Biological control; Microsatellites; High-throughput sequencing; Tea plantation; Stethynium empoasca

期刊名称：APPLIED ENTOMOLOGY AND ZOOLOGY （ 影响因子：1.403； 五年影响因子：1.381 ）

ISSN： 0003-6862

年卷期：

页码：

收录情况： SCI

摘要： Stethynium empoasca Subba Rao (Hymenoptera: Mymaridae) is an egg parasitoid of the predominant tea pest, Empoasca onukii Matsuda (Hemiptera: Cicadellidae), and is regarded as the most promising candidate for both augmentative and conservative biological control on E. onukii. However, little is presently known about its biology, ecology, and genetics. Here, we developed and characterized novel microsatellite markers for S. empoasca from transcriptome sequences generated using high-throughput sequencing. A total of 54,520 microsatellites were isolated from 117 Mb clean sequences. Fifty primers were developed and used to characterize 98 individuals from five geographically distinct populations, of which 18 loci were demonstrated to be polymorphic, stable, and repetitive. These loci also showed a low frequency of null alleles, suggesting little effect on their use in the estimation of genetic diversity and structure. Additionally, the assessed populations showed low to moderate levels of genetic differentiation, in which individuals clearly grouped into two clusters, indicating that the loci could reveal a pattern of spatial structure and gene flow in S. empoasca populations according to geographic variability. This work provides an important basis for future studies on how these markers can be used in studies of the biology, genetics, and ecology of this important parasitoid.

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